Mpox Virus Controls
- What are the Twist Synthetic Mpox Virus Controls?
- What are the concentrations of the mpox controls?
- How are the mpox controls qualified?
- What are the full sequences/breakpoints of the mpox controls?
- What is the storage temperature for the mpox controls?
- What fraction of the genome is covered by the mpox controls?
- Which variants are covered and how many fragments (and sizes) are in each control?
- What protocol do you recommend using with the mpox controls?
- What starting input would you recommend for the mpox controls?
- Why can’t I directly order the mpox control on your website?
- Does Twist make synthetic controls for other viruses?
- Are these controls ISO certified?
- Why is only 80-85% of the genome covered instead of 100%?
SARS-CoV-2 Controls
cfDNA Pan-cancer
- What is your background cfDNA made from?
- Could there be some mutation ‘contaminants’ coming from the background DNA as well?
- Is your background cfDNA limited supply?
- How do you perform quality control on the reference standards?
- Are these control oligos, RNA, what is in it/ components?
- How do you design the ctDNA/ variant list?
- What is the molecular structure of the 3′ and 5′ termini?
- Were the mutations in the standards confirmed by NGS as well, as with digital PCR as it says in the document?
- Recommendations for diluting the controls?
- Can’t find indel or other hard to detect variants?
- What reagents (e.g, library prep) do you use for the liquid biopsy workflow?
- What do you recommend for starting input?
- Do you recommend pooling this control with a patient sample after library prep, before NGS?
- The variant (COSM214499) is always high in 0% in NGS QC results, why?
- For NGS QC, do you use target capture sequence for the 456 variants, or use a whole genome sequence?
- 456 variants are all in one tube, or subsets?
- What are the kit configurations of the cfDNA standards?
