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Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results. 

Available configurations

Mechanical Fragmentation

This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Accommodates a wide range of DNA input types
  • Enables high-quality sequencing of low-quality samples
  • Minimizes start / stop site artifacts

Enzymatic Fragmentation

These kits incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Ideal for automated, high-throughput library preparation
  • Produces tunable, reproducible fragment sizes
  • Minimizes sequence bias, maximizes coverage depth

 

Overview of workflow application options

Based on your application needs, create sequencing ready libraries in as little as two hours.

NGS Library Preparation Workflow
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KEY BENEFITS

Accommodate Either Enzymatic or Mechanical Fragmentation

  • Use enzymatic fragmentation for automated, high-throughput gDNA
  • Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

  • Use with Twist full-length CD adapters for PCR-free library Preparation
  • Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

  • Optimized for use in the Twist Targeted NGS workflow
  • Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
  • Convert DNA samples into robust, amplified libraries in under 2.5 hours
  • Combine library preparation steps into a single reaction for improved efficiency and consistent results

New Library Preparation Kit

More options to achieve the quality data you need. Introducing Library Preparation Enzymatic Fragmentation Kit 2.0

Learn more

New Human Sample ID

Ensure the accuracy of whole exome sequencing by tracking samples from whole blood. Twist Human Sample ID utilizes established Twist Library Prep and multiplexed PCR to identify and track samples to prevent sample mix ups and incorrect data.

Learn More

Overview of the library preparation workflow

Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, adapter ligation and library amplification. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.


The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

library preparation workflow with Twist Universal Adapters.
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PRODUCT SKUS – ENZYMATIC FRAGMENTATION 1.0

104174

Twist Library Preparation EF Kit 1.0, Enzymatic Fragmentation, 16 Samples

104175

Twist Library Preparation EF Kit 1.0, Enzymatic Fragmentation, 96 Samples

PRODUCT SKUS – MECHANICAL FRAGMENTATION WITH AMPLIFICATION MIX

104176

Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples

104177

Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples

The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
PRODUCT SKUS – ENZYMATIC FRAGMENTATION 2.0

104206

Twist Library Preparation EF Kit 2.0, 16 Samples

104207

Twist Library Preparation EF Kit 2.0, 96 Samples

PRODUCT SKUS FOR THE TWIST NGS METHYLATION DETECTION SYSTEM

101976

NEBNext® EM-seq™ Kit for Twist Targeted Methylation Sequencing, 96 Samples

103557

Twist Methylation Enhancer, 12 Reactions

100578

Twist Universal Blocker, 12 Reactions

100983

Twist Binding and Purification Beads Kit, 12 Reactions

101174

Twist Fast Hybridization and Wash Kit, 12 Reactions

103496

Twist Targeted Methylation Sequencing Workflow, 96x12 Reactions (contains all reagents listed above)

2 reaction and 12 reaction sizes also available for enrichment.
Twist Custom Panels can be ordered separately. Please contact your sales representative for more information.

Twist NGS Library Preparation Kits
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Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
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Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
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Library Preparation EF 2.0 with Enzymatic Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow
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681 Gateway Blvd
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