Scalable, low-pass WGS for large studies

Accelerate whole genome sequencing library prep by processing high sample volumes. Prepare up to 960 samples with the Twist 96-Plex Library Prep Kit, significantly reducing per-sample costs. Designed for high-throughput applications such as rational drug design or industrial seed selection, this all-in-one system requires only 50 ng of input DNA to generate sequencing-ready libraries with full-length Illumina-compatible adapters.

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Efficiency at scale

Lower your cost per sample by processing multiple plates simultaneously. Minimize reagent overhead and plastic waste, and maximize your sequencing budget.

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Tunable performance

Optimize results with adjustable primer chemistry. Maintain high-quality data and uniform coverage regardless of the GC content of your specific targets.

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Simplified library prep

Generate up to 960 samples per kit using a streamlined, all-in-one reagent system. Move rapidly from input DNA to sequencer-ready libraries.

How it works

The Twist 96-Plex Library Prep workflow starts with up to 96 individual samples in a plate. After the first random priming and barcoding step, all samples are pooled into a single tube for the rest of the process. An optional plate barcode can be added in the index read position to allow simultaneous sequencing of multiple sample plates.

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GC content varies with different organisms, which can cause variable biases in library preparation. The Twist 96-Plex Library Prep Kit supplies two primer sets for high- and low-GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs.

Sequencing libraries prepared with the Twist 96-Plex Library Prep Kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.

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One kit for many sample types
Consistent libraries at scale

One kit for many sample types

GC content varies with different organisms, which can cause variable biases in library preparation. The Twist 96-Plex Library Prep Kit supplies two primer sets for high- and low-GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs.

One kit for many sample types

Consistent libraries at scale

Sequencing libraries prepared with the Twist 96-Plex Library Prep Kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.

Consistent libraries at scale
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One kit for many sample types

One kit for many sample types

GC content varies with different organisms, which can cause variable biases in library preparation. The Twist 96-Plex Library Prep Kit supplies two primer sets for high- and low-GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs.

Consistent libraries at scale

Consistent libraries at scale

Sequencing libraries prepared with the Twist 96-Plex Library Prep Kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.

Connecting plant trait genotypes to phenotypes using WGS skim-Seq

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