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- OVERVIEW
- TCR DATA
- ORDERING
- RESOURCES
Overview
Adoptive cell therapy (ACT) has led to highly effective cancer treatments for patients who had very few treatment options. It utilizes the patient’s own immune system to attack cancer cells.
Engineered TCR therapy is a type of ACT that leverages engineered T cell receptors to target tumor specific antigens. This starts with sequencing of the tumor biopsy to identify tumor mutations, and typically, the peripheral blood to uncover the TCR repertoire.
TCR repertoire sequencing can be done via single cell sequencing or bulk sequencing. Each has it’s own advantages. Bulk sequencing enables you to sample more of the sequence space but information about the alpha-beta TCR pairing is lost. Single-cell sequencing enables you to capture information on the alpha-beta chain pairing and receptor composition. However, single-cell sequencing has a much lower throughput than bulk sequencing.
How can Twist help me build TCR libraries?
Combinatorial TCR Library
- Enables you to shuffle alpha and beta chain pairs to create additional diversity and explore novel combinations beyond the identified repertoire
- This is a good complement to bulk sequencing where the alpha-beta TCR pairing is unknown
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Figure 1: Twist Combinatorial TCR Library with 4 defined alpha chains and 4 defined beta chains. Due to our combinatorial assembly, this will yield a library with 16 unique alpha and beta chain combinations. This provides all possible alpha and beta combinations rather than just a subset as described with arrayed libraries.
KEY BENEFITS
High Diversity and Quality
- Each combinatorial assembly can be composed of up to 10,000 gene fragment combinations
- NGS-verified libraries with > 90% of possible variants present within 10x of the mean
Customization
- Customize your TCR Library with user defined combinatorial variants across alpha and beta chains
- Customize your CAR Library with user defined combinatorial variants across hinge, transmembrane, and signaling domains
Flexibility with throughput
- Insert sequences up to 1.5 kb in length
- Libraries designed at scale with diversity across multiple elements of the sequence
Have questions?
Tell us what you would like more information on. We’re here to help!
Introducing the new Library Design Tool
Streamlined Library Design at Your Fingertips
Performance Data
The discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. TCR Libraries can be leveraged to rapidly generate combinations of TCR beta and alpha chains for high throughput screening. With combinatorial TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire.
Combinatorial TCR Libraries
Have questions?
Tell us what you would like more information on. We’re here to help!
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T Cell Receptor (TCR) Discovery workflow
Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR) libraries.
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We will provide you with highly uniform screening libraries that are precise, user-defined combinations of gene fragments, which enable you to efficiently test comprehensive screening of the desired combinations.
A New Way of Designing Your Libraries
The New Library Design Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.
Expert Support Still at Your Fingertips
If you have any questions, please feel free to email us at library@twistbioscience.com
Design Companion
- Intuitive, interactive portal to easily upload your library sequence file
- Identify potential errors in sequence with instant feedback enabling library validation on the fly
- Save drafts for future reference
Real-time Optimization
- Errors are flagged for review, letting you quickly resolve issues without delays
- Our library design experts are still ready to assist you with any questions
- With our intuitive tool and expert support we can help you find the fastest path to a validated, high-quality library
Faster Process
- Real-time, interactive library design interface supports optimizing your library design and cost efficiently
- Save drafts for future reference
- Automatically generates a Statement of Work (SOW) for your order, expediting the entire ordering process
Watch Step by Step how to optimize your sequences
START DESIGN NOW
Let’s get Started
The New Digital Library Design (DLD) Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.
Step 1
Log into your account. If you don’t have one, it only takes a few minutes to set it up!
Step 2
Start to design your library in the new interactive libraries design tool which has a step by step “how to” wizard. With this option you can optimize your library in real time with validations on the fly and real-time pricing and receive an SOW at the end of the submission process.
Step 3
Once the quote is accepted by the customer, the Libraries Team will send the project to the production team
Note:
The Libraries Design Tool is not currently available in the APAC region. Please check with your local support team on availability date.
Combinatorial Assembly Libraries Product Brochure
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
