Oligo pools, high diversity collections of oligonucleotides, are utilized in many applications, including generation of CRISPR guide RNA (sgRNA) libraries and high-throughput reporter assays. Accuracy and uniformity of oligo synthesis are critical to ensure the oligo pools generated precisely match the design. Oligo pools for CRISPR screens are cloned into expression vectors, generating sgRNA libraries. Our industry-leading low synthesis error rate and uniform sequence representation result in high quality custom oligo libraries that enable the most efficient CRISPR screens.