All 456 variants are confirmed by NGS (for both 0% and 5% VAF), and the 0.1%, 0.25%, 0.5%, 1% and 2% are generated from a blending of 0% and 5% stocks. We selected a set of six representative and relevant variants  across 0% to 5% VAFs for ddPCR, showing quantitative formulations and a continuous serial dilution.