Custom Library Design and Generation

Precision Library Synthesis, No Compromises

Overview
BENEFITS
Data

High-Diversity Libraries, Expertly
Constructed for Your Target

Traditional custom library construction methods can result in redundant codons, unpredictable diversity, and non-uniform amino acid distribution.

With base-by-base precision, our silicon-based DNA synthesis platform can accurately control library diversity, ratio of amino acid distribution, CDR length variation, and codon usage enabling the construction of high-quality bespoke libraries, focused on the most promising sequence space.

A Customizable Discovery Engine, Fit for Your Needs

Focus on the Library and Sequence Space of Your Choosing

We enable our partners to reach their R&D goals with unparalleled efficiency with a multi-faceted custom library approach. From various starting points (e.g. immunizations, PBMCs, databases, etc.), we can construct immunized, combinatorial variants, or assembly (VH/VL shuffle) libraries suitable for your antibody discovery program. Our Binary Substitution Libraries offer precise humanization by minimizing immunogenicity risks while maximizing therapeutic potential.

We also offer substitution libraries that employ a traditional method that biases the ratio towards wild-type amino acids, thus limiting complexity at each variant position, and limiting the combinatorial substitutions from the wild-type. For those requiring even greater precision, our individual substitution library allows for user-defined compositions with higher-order substitutions based on domain diversity.

Access Derisked, Liability-Free Libraries

To help you avoid downstream issues, we remove all amino acid sequences associated with developability and manufacturability complications. For all of our custom libraries, we can selectively remove motifs associated with glycosylation, asparagine deamidation, aspartate isomerization, lysine glycation, CD11c/CD18 binding, fragmentation, and hydrophobicity.

Oligo pools can be designed tomatch the natural CDR repertoire

Liabilities can be removed, e.g. isomerization, cleavage, deamidation, glycosylation sites

Rational sampling from desired sequence space

Accurate representation, e.g. motif sequences can be explicitly encoded in oligos

NGS Validation for Better QC

Each of our custom libraries is validated by next-generation sequencing (NGS) to determine the variants present prior to selection. We ensure that we achieve greater than 105 read coverage to ensure full diversity incorporation and uniform distribution of variants.