Spread Out Low Diversity Libraries

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The Latest Tool for Mapping Protein Sequences

Spread-Out Low Diversity (SOLD) libraries provide a quick and precise tool for mapping protein sequences to explore the complex relationship between a protein and its environment.

SOLD Libraries are a time- and money-saving tool for researchers who want to efficiently investigate combinatorial possibilities.

A library of precise combinatorial variants with uniform amino acid distribution, balanced codon usage and the ability to avoid unwanted restriction sites, provides the ultimate tool to explore the variant space. All SOLD Libraries are NGS-verified, cloning-ready, and created using Twist’s patented silicon-based synthesis platform, ensuring low error rates.

Flexibility

Greater flexibility than the traditional approach
Suitable for sites with scattered diversity
No template required

Precision

No premature stop codons or unwanted codons
Precise amino acid and codon control
More precise than traditional methods, e.g. NNK, TRIM and epPCR.

Superior Quality

SOLD libraries include NGS verification of modified regions
Rigorous quality control and verification of all variants
Uniform distribution of amino acids at each variant position

Explore Spread Out Low Diversity Libraries

Learn how the SOLD Libraries enable the exploration of the sequence space encoded by mutating multiple positions simultaneously scattered across the parental sequence to generate a diverse library of proteins.

Introducing the new Library Design Tool

Streamlined Library Design at Your Fingertips

A High-Fidelity Synthetic Construct

Twist’s SOLD Libraries have shown their ability to consistently deliver amino acid distributions that closely match the requested amino acid frequencies, with a uniform distribution of variants and no dropouts

SOLD Library: Diversity is scattered along the WT sequence

Twist's SOLD Libraries have the benefit of precisely incorporating diversity across a wild-type sequence without restricting that diversity to small variant domains. This ability to simultaneously investigate multiple amino acid positions across a sequence allows researchers to investigate, and optimize, the activity of a protein both rapidly and effectively

A New Way of Designing Your Libraries

The New Library Design Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

START DESIGN NOW

Design Companion

Intuitive, interactive portal to easily upload your library sequence file
Identify potential errors in sequence with instant feedback enabling library validation on the fly
Save drafts for future reference

Real-time Optimization

Errors are flagged for review, letting you quickly resolve issues without delays
Our library design experts are still ready to assist you with any questions
With our intuitive tool and expert support we can help you find the fastest path to a validated, high-quality library

Faster Process

Real-time, interactive library design interface supports optimizing your library design and cost efficiently
Save drafts for future reference
Automatically generates a Statement of Work (SOW) for your order, expediting the entire ordering process

Expert Support Still at Your Fingertips

If you have any questions, please feel free to email us at library@twistbioscience.com

Take a peek at our Library Design Tool

A New Way of Designing Your Libraries at Twist

Watch Step by Step how to optimize your sequences

START DESIGN NOW

Let’s get Started

The New Digital Library Design (DLD) Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

Step 1

Log into your account. If you don’t have one, it only takes a few minutes to set it up!

Step 2

Start to design your library in the new interactive libraries design tool which has a step by step “how to” wizard. With this option you can optimize your library in real time with validations on the fly and real-time pricing and receive an SOW at the end of the submission process.