Mechanical Fragmentation Library Prep Kit

OVERVIEW

Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.

Twist Library Preparation Kit (Mechanical Fragmentation) is designed for use
with gDNA that has already been sheared mechanically. It combines the enzymes
and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

Accommodates a wide range of DNA input types
Enables high-quality sequencing of low-quality samples
Minimizes start / stop site artifacts

KEY BENEFITS

Accommodate Either Enzymatic or Mechanical Fragmentation

Use enzymatic fragmentation for automated, high-throughput gDNA library construction

Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

Use with either Twist full-length CD adapters for PCR-free library preparation

Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

Optimized for use in the Twist Targeted NGS workflow

Flexible DNA sample input from 1 ng – 1 µg (enzymatic)

Convert DNA samples into robust, amplified libraries in under 2.5 hours

Combine library preparation steps into a single reaction for improved efficiency and consistent results

Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.

Twist Library Preparation Kit (Mechanical Fragmentation) is designed for use
with gDNA that has already been sheared mechanically. It combines the enzymes
and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

Accommodates a wide range of DNA input types
Enables high-quality sequencing of low-quality samples
Minimizes start / stop site artifacts

KEY BENEFITS

Accommodate Either Enzymatic or Mechanical Fragmentation

Use enzymatic fragmentation for automated, high-throughput gDNA library construction

Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

Use with either Twist full-length CD adapters for PCR-free library preparation

Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

Optimized for use in the Twist Targeted NGS workflow

Flexible DNA sample input from 1 ng – 1 µg (enzymatic)

Convert DNA samples into robust, amplified libraries in under 2.5 hours

Combine library preparation steps into a single reaction for improved efficiency and consistent results

DATA

Twist’s Rationally Sculpted Libraries Are Unparalleled in Quality and Composition

Comparison of Combinatorial Variant Library GenerationMethods

Twist’s Rationally Sculpted Libraries Are Unparalleled in Quality and Composition

Comparison of Combinatorial Variant Library GenerationMethods

ORDERING & SPECS

PRODUCT SKUS

101280: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples

101281: Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples

Reagents, enzymes, and purification beads required for the library construction

of mechanically fragmented gDNA.

PRODUCT SKUS

101280: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples

101281: Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples

Reagents, enzymes, and purification beads required for the library construction

of mechanically fragmented gDNA.

RESOURCES

Product Sheet