Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.
Twist Library Preparation Kit (Mechanical Fragmentation) is designed for use
with gDNA that has already been sheared mechanically. It combines the enzymes
and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.
- Accommodates a wide range of DNA input types
- Enables high-quality sequencing of low-quality samples
- Minimizes start / stop site artifacts
KEY BENEFITS
Accommodate Either Enzymatic or Mechanical Fragmentation
Use enzymatic fragmentation for automated, high-throughput gDNA library construction
Use mechanical fragmentation for DNA input of varying types and quality
Accommodate Either Whole Genome Sequencing or Targeted Enrichment
Use with either Twist full-length CD adapters for PCR-free library preparation
Use with the Twist Universal Adapter (UDI) System for maximum yield
Streamline Your Workflow
Optimized for use in the Twist Targeted NGS workflow
Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
Convert DNA samples into robust, amplified libraries in under 2.5 hours
Combine library preparation steps into a single reaction for improved efficiency and consistent results
Product Sheet
Protocol
