Mpox Virus Controls

The mpox sequences are to be provided upon request. Contact customersupport@twistbioscience.com for inquiries.

The mpox controls are validated and they are compatible with CDC protocols. The CDC protocols can be found here.

There are two Clades: Congo Basin Clade (Control 1) 

West Africa Clade (Control 2)  

Control 1 contains 154 fragments while Control 2 contains 151 fragments. Each of the fragments are around 1.8kb.

Each control covers 80-85% of the mpox virus genome.

Each of the mpox controls contains 100 µL at 1x10^5 copies/ µL fragments.

Twist provides a suite of research tools and NGS products, including custom controls

and reference standards for mpox, respiratory diseases including coronaviruses,

influenza viruses, paramyxoviruses and enteroviruses as well as liquid biopsy and cancers. 

If your product of interest is not included in our offering, like panels or controls, please contact your sales manager or customer support at customersupport@twistbioscience.com.  We will help you with potential custom solutions.

Our mpox virus controls are not ISO certified. To respond quickly to the public health need, we decided to launch the controls without an ISO certification.  However, we are open to getting these SKUs ISO certified.  Please let us know if you are interested in ISO certification.

The controls are shipped frozen in dry ice. Storage temperature is recommended at -80°C, but the controls can be stored at -20°C for short term storage.

Repetitive sequences and areas of extremely high or low GC content as well as homopolymers  are excluded in the genome.  It’s challenging to target those regions.

Since the mpox controls are double-stranded DNA controls, any purchase outside the US requires an export license due to the biosecurity concern. For US-based shipping, an export license will not be needed but please work with your NGS Account Manager to generate a quote and place an order.  If you are based outside of the US, please contact Twist customer support (customersupport@twistbioscience.com)  and provide your name and institution’s name and address.  Our customer support team will work with you to get your export license granted.

We recommend starting with 1 uL per reaction and adjusting usage based on the results.

The Twist Synthetic human mpox controls have a concentration within 50% to 200% of the targeted 100,000 copies/ul as determined by ddPCR (T1 assay), or between 50,000 to 200,000 copies/µL.

The Certificate of Analysis document reports out at least 100,000copies/µL fragments concentration with a range of 25% to 400%, or 25,000 - 400,000 copies/µL per fragments.

Twist Synthetic hMPXV Control 1 (EPI_ISL_13056236) 106051 (component) / 106056 (finished good)

Twist Synthetic hMPXV Control 2 (NCBI:ON585037) 106055 (component) / 106059 (finished good)

The mpox sequences are to be provided upon request. Contact customersupport@twistbioscience.com for inquiries.

The mpox controls are validated and they are compatible with CDC protocols. The CDC protocols can be found here.

There are two Clades: Congo Basin Clade (Control 1) 

West Africa Clade (Control 2)  

Control 1 contains 154 fragments while Control 2 contains 151 fragments. Each of the fragments are around 1.8kb.

Each control covers 80-85% of the mpox virus genome.

Each of the mpox controls contains 100 µL at 1x10^5 copies/ µL fragments.

Twist provides a suite of research tools and NGS products, including custom controls

and reference standards for mpox, respiratory diseases including coronaviruses,

influenza viruses, paramyxoviruses and enteroviruses as well as liquid biopsy and cancers. 

If your product of interest is not included in our offering, like panels or controls, please contact your sales manager or customer support at customersupport@twistbioscience.com.  We will help you with potential custom solutions.

Our mpox virus controls are not ISO certified. To respond quickly to the public health need, we decided to launch the controls without an ISO certification.  However, we are open to getting these SKUs ISO certified.  Please let us know if you are interested in ISO certification.

The controls are shipped frozen in dry ice. Storage temperature is recommended at -80°C, but the controls can be stored at -20°C for short term storage.

Repetitive sequences and areas of extremely high or low GC content as well as homopolymers  are excluded in the genome.  It’s challenging to target those regions.

Since the mpox controls are double-stranded DNA controls, any purchase outside the US requires an export license due to the biosecurity concern. For US-based shipping, an export license will not be needed but please work with your NGS Account Manager to generate a quote and place an order.  If you are based outside of the US, please contact Twist customer support (customersupport@twistbioscience.com)  and provide your name and institution’s name and address.  Our customer support team will work with you to get your export license granted.

We recommend starting with 1 uL per reaction and adjusting usage based on the results.

The Twist Synthetic human mpox controls have a concentration within 50% to 200% of the targeted 100,000 copies/ul as determined by ddPCR (T1 assay), or between 50,000 to 200,000 copies/µL.

The Certificate of Analysis document reports out at least 100,000copies/µL fragments concentration with a range of 25% to 400%, or 25,000 - 400,000 copies/µL per fragments.

Twist Synthetic hMPXV Control 1 (EPI_ISL_13056236) 106051 (component) / 106056 (finished good)

Twist Synthetic hMPXV Control 2 (NCBI:ON585037) 106055 (component) / 106059 (finished good)