High Throughput
Antibody Production

The Power of Silicon-based DNA Synthesis

Custom antibody products in HEK293 can be used to help relieve bottlenecks in times of capacity constraints. Let Twist Express Antibodies, HEK293, be an extension of your lab to provide you with fast, high-quality, purified antibodies ready for screening. Twist Express Antibodies produced in Expi293™ cells, are harvested and either filtered (Supernatant) or purified using Protein A. The antibodies are then transferred to a preferred shipping container, frozen, and delivered along with the Glycerol Stocks of the individual chains in perfect shape for any functional assay or screening application.

• High-quality antibodies produced from NGS sequence-verified Twist Express Clonal Genes 
• Ready to use and scale-up ready using included Glycerol Stock

Twist Express Antibodies can be easily ordered using our integrated eCommerce application with just the Variable (VH & VL) sequences. Please read through our Quick Start Guide for ordering tips. The antibodies can also be thawed using our handling guidelines (here)  for use in any functional or screening assays. 

Workflow

We've enhanced our antibody offering by leveraging Express Genes for reduced TAT, expanding cell-line offerings and add-ons including normalization, endotoxin testing, and aliquoting to meet your protein production and purification needs.

Performance Data

Figure Description: Following antibody production, antibodies were purified and yields were determined. Of the 518 antibodies produced, 482 passed size and purity QC tests-409 yielded more than 100 ug per antibody.

Twist Express Antibodies, 293 Product Offering

Resources and Guidance for Sequence Design

1.  abYsis provides an integrated, comprehensive, and annotated sequence assessment for one or multiple antibodies. abYsis provides a thorough regional breakdown of antibody sequences to quickly identify where your variable region starts and ends. A tabular breakdown of the leader, FWK, and CDR sequences as well as the tail, or constant region sequence is also provided.

2. It can be helpful to apply the annotations provided by abYsis to your sequence using bioinformatic programs to help visualize and isolate your variable region. Below is an example of a sequence imported to the Geneious Prime software platform with abYsis annotations applied.

   

3. One additional option that may provide comprehensive breakdown of leader, variable, and constant regions is ANARCI. The resulting analysis provides the exact variable region sequence highlighted in red, which can then be uploaded to the Antibodies application on our eCommerce website. ANARCI also provides detail on the light chain domain type and the sequence species.

4. Please keep in mind that variable region sequences should never start with a methionine (M). If you are uploading sequences that start with the amino acid M, or nucleotides ATG, and are planning to use pTwist vectors, please contact our support for assistance.

5. Reformatting variable domains from one species to another can be complicated given differences between disulfide bonding and other features. By default, the Twist eCommerce platform will not correct any of these issues. We typically rely on you to account and inspect for these types of reformatting complications. If you need assistance, please contact our support team.