What are the lengths available for Multiplexed Gene Fragments?
- Fragment length can be between 301-500 nt (including constant flanks).
- The total length of the fragment cannot exceed 500 nucleotides including constant flanks which will be used for PCR amplification. Flanking sequences must be at least 20-25 bps in length, which will allow for up to 460 bps of variable coding region.
- The variation of fragment lengths from shortest to longest should not exceed 80 bp (e.g. if the longest fragment is 400 bp, the shortest fragment should be > 320 bp).
Was this article helpful?
Yes
No
Still have questions? Contact Us
