Robust chemistry for challenging samples

Twist RNA-Seq solutions, designed for demanding samples such as low input and FFPE so you can get high quality libraries with high yield in a short workflow so you can get results. Twist offers both Target Enrichment and Whole Transcriptome workflows that offer options to best suit your application needs. Each workflow delivers high quality libraries ready for sequencing from a wide range of inputs and sample types, including translational research samples.

Choose your workflow

Target Enrichment

BENEFITS

  • Capture all genes or subsets of genes of interest
  • Enrich damaged RNA fragments from FFPE
  • Enrich full length transcripts of expressed genes
  • Create your own custom RNA panels

Specifications

infi Include FFPE repair module 
bar 1 - 1000 ng 
time Library Prep:  4.5 - 5 hrs
mag Enrichment: Overnight (16 hrs) with Standard Hyb
v2 or 5 hrs with Fast Hyb and Wash

 

Product offering

RNA Exome Panel

Gene expression analysis with coverage of mRNA sequences including novel gene fusions 

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RNA Fusion Panel

Focused analysis of known and novel fusion events in oncogenic genes 

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RNA Custom Panel

Enrichment of unique target list of expressed RNAs 

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Twist Targeted RNA Library Prep

RNA library preparation for target enrichment workflows

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Whole Transcriptome

BENEFITS

  • Unbiased profiling of expressed RNAs
  • FFPE compatible
  • rRNA and globin gene depletion
  • Fast workflow

Specifications

infi Include FFPE repair module 
bar 1 - 1000 ng 
time Library Prep:  4.5 - 5 hrs (6 - 6.5 hrs with depletion module)
mag Enrichment: N/A 

 

Product offering

Twist Whole Transcriptome RNA Library Prep

Ideal for deep transcriptome profiling of samples from tissues or cells

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Twist Whole Transcriptome RNA Library Prep with Depletion

Unbiased library preparation which includes rRNA and globin transcript depletion

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Need help deciding on a workflow?

talk to a scientist

What Scientists Have to Say

 

 

 

 

“

The transcriptome provides closer access to the real biological status of the tumour cell, in contrast to the more ‘static’ nature of DNA. The parallel analysis of gene expression, fusion detection, variant calling, tumour signatures, and much more, possible with one library prep and starting from three to five FFPE tissue slices makes the RNA exome kit a promising assay for future-proof implementation into our clinical routine.

”
Dr. Koen Jacobs, Molecular Pathologist, AZ Sint Lucas

Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.

“

Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 fusion variants in a high-throughput lab workflow.

”
Dr. Florian Battke, Director of Development, CeGaT GmbH

Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.

Featured Resources

Twist RNA Workflows Product Sheet
Product Sheet NGS
Twist RNA Exome Product Sheet
Product Sheet NGS
Evaluation of a targeted RNA sequencing workflow for variant calling from FFPE samples in NSCLC
Application Note NGS

FAQ

The Twist RNA Exome is a Target Enrichment which provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. Additionally, the RNA Exome’s “exon-aware” design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches.

The depletion module is designed to work with blood containing samples or otherwise samples which may benefit from the removal of contaminating rRNA and globin.

The FFPE repair module is an optional included step in the library preparation which aids in decrosslinking of DNA from formalin fixed making those sequences more readily available to participate in the subsequent library generation.

Yes, Twist’s expert bioinformatics team can create panels to across many different organisms, such as microbes, or for a variety of applications.

Ready to discuss improving your RNA sequencing?

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