Twist 96-Plex Library Prep Kit

Overview

Preparing DNA sequencing libraries often involves complex workflows and only produces small batches of samples at a time. For genomic studies where hundreds of specimens are being evaluated, cost-effective tools that can be integrated with existing processes are essential.

The Twist 96-Plex Library Preparation kit enables high-throughput library construction for whole genome sequencing. The kit is purpose-built for use in high throughput applications, including industrial seed selection, cattle breeding, and rational drug design. With primer tunability for varying GC content and up to 960 samples per kit, the 96-Plex Library Preparation kit offers an optimal balance between scalability and robust library construction.

Workflow

The Twist 96-Plex Library Preparation workflow starts with up to 96 individual samples in a plate. After the first random priming and barcoding step, all samples are pooled into a single tube for the rest of the process. An optional plate barcode can be added in the index read position to allow simultaneous sequencing of multiple sample plates.

Twist 96-plex Library Prep Workflow

Recommended Application

Low-pass sequencing
Industrial seed selection
Cattle breeding
Plasmid sequence verification

Kit Overview

The Twist 96-Plex Library Preparation kit contains all reagents needed to prepare double stranded libraries with full length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight input material. No additional primers, enzymes, or beads are required.

The kit is available in 960 sample format, containing sufficient materials for five or ten 96-sample pooled batches.

KEY BENEFITS

High throughput

Up to 960 individually barcoded samples per kit

Highly multiplexed reactions for up to 96 samples at once

Efficient

$10 per sample

No fragmentation, repair, or ligation steps necessary

Adaptable

Enables optimization for high- or low-GC content samples

Same kit can be used for multiple sample types

Economical solution for hundreds of samples

At just $10 per sample, the Twist 96-Plex Library Preparation kit scales up without breaking the bank.
Each preparation process produces 96 sequencer-ready libraries that seamlessly integrate with existing
sequencing workflows.

Webinar

Connecting Plant Trait Genotypes to Phenotypes using WGS Skim-Seq.

Jason Johns

Testimonial

“To be able to pool all of the samples to with 5 tubes instead of almost 500 different wells was really nice!”

Jason Johns,

Researcher UC Santa Barbara

Twist 96-plex High Throughput NGS Library prep

Preparing DNA sequencing libraries often involves complex workflows and only produces small batches of samples at a time. For genomic studies where hundreds of specimens are being evaluated, cost-effective tools that can be integrated with existing processes are essential.

The Twist 96-Plex Library Preparation kit enables high-throughput library construction for whole genome sequencing. The kit is purpose-built for use in high throughput applications, including industrial seed selection, cattle breeding, and rational drug design. With primer tunability for varying GC content and up to 960 samples per kit, the 96-Plex Library Preparation kit offers an optimal balance between scalability and robust library construction.