Multiplexed Gene Fragments 

Introducing Twist Multiplexed Gene Fragments

Twist Multiplexed Gene Fragments are double stranded gene fragment sequences of up to 500 base pairs in length that are delivered as a pool instead of one fragment per well matrix. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.
Multiplexed Gene Fragments are synthesized from user defined sequences in lengths of up to 500 base pairs per gene. These user-defined sequences are initially synthesized into oligo pools of up to 500 nucleotides in length and are then amplified in double-stranded DNA, and delivered as multiplexed gene fragments that have been carefully analyzed to ensure >90% of genes in the pool are the correct length. This product is optimized for rapid, high-throughput screening applications.

*Terms and Conditions: Standard turnaround time for Multiplexed Gene Fragments is 8-12 business days. This will vary based on sequence length and complexity. Multiplexed Gene Fragment length is up to 500 base pairs, with up to 460 bp allotted for the variable region with a minimum of 40 bp conserved for the primer region. Quality control tests ensure >90% of gene sequences within a pool will be user defined length.

For research use only. Not for Diagnostic Procedures.