Structural scFv Library

The Twist Structural scFv Library is a synthetic antibody library that incorporates the CDR sequences from 3,700 antibodies with known crystal structures from a protein structure database. To improve manufacturability, liabilities such as unpaired C- and N-glycosylation, deamination, and hydrolysis sites are eliminated. The heavy chain (VH) library shuffles 148 unique CDR1s, 151 unique CDR2s, and 564 unique CDR3s into the human IGHV3-23 framework. The light chain (VL) library shuffles 134 unique CDR1s, 158 unique CDR2s, and 278 unique CDR3s into the human IGKV1-39 framework. When combined, the VH and VL libraries yield a fully human scFv library with a diversity of 4 x 10¹⁰.

Go from panning to functional assays in 10–12 weeks. The process starts with phage screening the diverse Twist Structural scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Structural scFv Library to initiate your own in-house discovery projects. To learn more, get in touch at biopharma@twistbioscience.com.

Titration ELISA shows the cross-reactivity of TB138-6 with human and cynomolgus monkey CD3.

Flow titration demonstrates that TB138-6 binds CD3+ cells (CD8+ T-cell) and not CD3- cells (CHO-GLP1R).