Carbohydrate scFv Library

Library Specifications

The Twist Carbohydrate scFv Library combines heavy chain (VH) and light chain (VL) libraries to yield a fully human scFv library of 2 x 109 size. The heavy chain design shuffles 56 unique HCDR1s and 65 unique HCDR2s in the context of the human IGHV3-23 framework. The light chain design incorporates 62 unique LCDR1s and 47 unique LCDR2s in the context of the human IGKV4-1 framework. The CDR3 regions derive their diversity from 52 structures of antibodies in complex with carbohydrate antigens and are biased towards incorporating residues that make up the carbohydrate-antigen interface. These CDR3 regions include both positively and negatively charged amino acids, as observed in the 130 carbohydrate-binding antibodies from which this library is derived.

Library Panning & Screening Process

Go from panning to functional assays in 10–12 weeks. The process starts with phage screening the diverse Twist Minotaur scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Minotaur scFv library to initiate your own in-house discovery projects. To learn more, get in touch at biopharma@twistbioscience.com