Carbohydrate scFv Library

The Twist Carbohydrate scFv Library combines heavy chain (VH) and light chain (VL) libraries to yield a fully human scFv library of 2 x 10⁹ size. The heavy chain design shuffles 56 unique HCDR1s and 65 unique HCDR2s in the context of the human IGHV3-23 framework. The light chain design incorporates 62 unique LCDR1s and 47 unique LCDR2s in the context of the human IGKV4-1 framework. The CDR3 regions derive their diversity from 52 structures of antibodies in complex with carbohydrate antigens and are biased towards incorporating residues that make up the carbohydrate-antigen interface. These CDR3 regions include both positively and negatively charged amino acids, as observed in the 130 carbohydrate-binding antibodies from which this library is derived.

Go from panning to functional assays in 10-12 weeks. The process starts with phage screening the diverse Twist Carbohydrate scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Carbohydrate scFv library to initiate your own in-house discovery projects. To learn more, get in touch at biopharma@twistbioscience.com.