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Protocol

Twist Library Preparation Protocol — Mechanical Fragmentation and Combinatorial Dual Indices

This Twist Library Preparation Kit provides the reagents needed to prepare genomic DNA (gDNA) libraries using mechanically fragmented DNA and Y-shaped adapters. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.