Do Twist Cloning or Expression vectors come with a Kozak and signal peptide sequence included in the vector backbone?
The only Twist-provided vector that contains a Kozak sequence is pTwist ENTR Kozak. All remaining Cloning, Expression, and antibody backbone vectors offered by Twist Bioscience DO NOT contain Kozak or signal peptide (leader) sequences. Twist recommends including a Kozak (GCCGCCACC or variant) along with a signal sequence when planning to express proteins in Mammalian expression systems.
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