Trinity Workflow:
The traditional hybrid selection process for targeted panels, like whole exome panels, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.
With the simplified Trinity workflow, you’ll hybridize your DNA library to the target probes of interest, incubate with the panel probes for as little as one hour with our fast hyb reagents, and load it directly on your AVITI sequencer. This drastically reduces manual steps and additional amplification resulting in higher library complexity and lower duplication rates.
Request additional information about how to streamline your whole exome sequencing workflows on Element Bio’s AVITI system.
Trinity Sequencing: Library preparation and Target Enrichment Workflow
Overview of Trinity workflow as compared to the traditional Twist library preparation and target enrichment protocol.
Trinity Workflow:
The traditional hybrid selection process for targeted panels, like whole exome panels, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.
With the simplified Trinity workflow, you’ll hybridize your DNA library to the target probes of interest, incubate with the panel probes for as little as one hour with our fast hyb reagents, and load it directly on your AVITI sequencer. This drastically reduces manual steps and additional amplification resulting in higher library complexity and lower duplication rates.
Request additional information about how to streamline your whole exome sequencing workflows on Element Bio’s AVITI system.
Trinity Sequencing: Library preparation and Target Enrichment Workflow
Overview of Trinity workflow as compared to the traditional Twist library preparation and target enrichment protocol.
Element Bioscience’s AVITI platform offers high-quality sequencing with the advantages of a flexible platform. The AVITI platform offers dual independent flow cells for flexibility in run configuration and throughput to suit the particular needs of a laboratory. This allows laboratories to avoid batching samples, which reduces costs. Additionally, with the use of Element’s Cloudbreak chemistry, users can save time and protocol steps by eliminating the need to convert linear libraries to circularized libraries.
Twist now offers a complete native exome enrichment workflow which includes our most complete and inclusive exome panel paired with our high-performing library preparation and enrichment reagents for a complete solution to go from sample to sequencing on the AVITI platform. This workflow incorporates adapters designed to be coupled with Element’s Cloudbreak sequencing chemistry. This workflow also enables users to customize content by spiking their own content to the exome panel. Twist’s workflow solution for exome sequencing is an all-in-one solution with ensured compatibility of reagents for AVITI sequencing.
Element Bioscience’s AVITI platform offers high-quality sequencing with the advantages of a flexible platform. The AVITI platform offers dual independent flow cells for flexibility in run configuration and throughput to suit the particular needs of a laboratory. This allows laboratories to avoid batching samples, which reduces costs. Additionally, with the use of Element’s Cloudbreak chemistry, users can save time and protocol steps by eliminating the need to convert linear libraries to circularized libraries.
Twist now offers a complete native exome enrichment workflow which includes our most complete and inclusive exome panel paired with our high-performing library preparation and enrichment reagents for a complete solution to go from sample to sequencing on the AVITI platform. This workflow incorporates adapters designed to be coupled with Element’s Cloudbreak sequencing chemistry. This workflow also enables users to customize content by spiking their own content to the exome panel. Twist’s workflow solution for exome sequencing is an all-in-one solution with ensured compatibility of reagents for AVITI sequencing.
Element Cloudbreak Compatible Workflow Kits:
107685: Twist for Element, Exome 2.0 + Comp Spike workflow, including Enzymatic Fragmentation 1.0 +Standard Hyb +Twist Universal Adapter System, 16 Samples – Element Compatible, 2 reactions
107686:Twist for Element, Exome 2.0 + Comp Spike workflow, including Enzymatic Fragmentation 1.0 +Standard Hyb +Twist Universal Adapter System, 16 Samples – Element Compatible, 2 reactions
109326*: 96 sample Twist for Element Trinity, Exome 2.0 + Comp Spike, Standard Hyb Workflow Reagents for constructing libraries and performing standard hybridization. The resulting enriched libraries are compatible with Element Trinity workflows.
109328*: 96 sample Twist for Element Trinity, Exome 2.0 + Comp Spike, Fast Hyb Workflow Reagents for constructing libraries and performing fast hybridization. The resulting enriched libraries are compatible with Element Trinity workflows.
Trinity Compatible Workflow Kits:
107678 Twist Universal Adapter System, 16 Samples – Element Compatible • Twist Universal Adapters - Element Compatible • Twist UDI Primers - Element Compatible–20°C
107682 Twist Universal Adapter System, 96 Samples – Element Compatible • Twist Universal Adapters - Element Compatible • Twist UDI Primers - Element Compatible–20°C
*All AVITI Compatible reagents available for individual purchase.
For research use only, use of Twist’s website and products are subject to Twist’s Terms and Conditions.
Element Cloudbreak Compatible Workflow Kits:
107685: Twist for Element, Exome 2.0 + Comp Spike workflow, including Enzymatic Fragmentation 1.0 +Standard Hyb +Twist Universal Adapter System, 16 Samples – Element Compatible, 2 reactions
107686:Twist for Element, Exome 2.0 + Comp Spike workflow, including Enzymatic Fragmentation 1.0 +Standard Hyb +Twist Universal Adapter System, 16 Samples – Element Compatible, 2 reactions
109326*: 96 sample Twist for Element Trinity, Exome 2.0 + Comp Spike, Standard Hyb Workflow Reagents for constructing libraries and performing standard hybridization. The resulting enriched libraries are compatible with Element Trinity workflows.
109328*: 96 sample Twist for Element Trinity, Exome 2.0 + Comp Spike, Fast Hyb Workflow Reagents for constructing libraries and performing fast hybridization. The resulting enriched libraries are compatible with Element Trinity workflows.
Trinity Compatible Workflow Kits:
107678 Twist Universal Adapter System, 16 Samples – Element Compatible • Twist Universal Adapters - Element Compatible • Twist UDI Primers - Element Compatible–20°C
107682 Twist Universal Adapter System, 96 Samples – Element Compatible • Twist Universal Adapters - Element Compatible • Twist UDI Primers - Element Compatible–20°C
*All AVITI Compatible reagents available for individual purchase.
For research use only, use of Twist’s website and products are subject to Twist’s Terms and Conditions.
Product Sheet
Data Files
Data Files
Twist for Element Unique Dual Index Sequences; reference spreadsheets and sample sheet templates
Protocol
Protocol
Twist for Element Exome 2.0 + Comprehensive Exome Spike-in Library Preparation and Fast
Hybridization With Trinity™ Sequencing Workflow
Protocol
Quick Guide for the Trinity Workflow: Twist Standard Hybridization & Trinity Run Setup
Protocol
Quick Guide for the Trinity Workflow: Twist Fast Hybridization & Trinity Run Setup
Protocol
Twist for Element Exome 2.0 + Comprehensive Exome Spike-in Library Preparation and Standard Hybridization With Trinity™ Sequencing Workflow
Data Files
Twist for Element Unique Dual Index Sequences; reference spreadsheets and sample sheet templates
Protocol
Twist for Element Exome 2.0 + Comprehensive Exome Spike-in Library Preparation and Fast
Hybridization With Trinity™ Sequencing Workflow
Protocol
Quick Guide for the Trinity Workflow: Twist Standard Hybridization & Trinity Run Setup
Protocol
Quick Guide for the Trinity Workflow: Twist Fast Hybridization & Trinity Run Setup
Protocol
Twist for Element Exome 2.0 + Comprehensive Exome Spike-in Library Preparation and Standard Hybridization With Trinity™ Sequencing Workflow
NEW Trinity Compatible Exome Workflows
