The Twist Hyperimmune Library synthetically mimics the human in vivo antibody repertoire, providing optimal diversity for antibody development against any target.
This naïve library is the original design of the Twist Hyperimmune Library series, which also includes the VHH hShuffle Hyperimmune (HI) and Common Light Chain Hyperimmune libraries.
Hyperimmune Fab Library
A highly diverse kappa VK1-39 light chain library was paired with the VH3-23 heavy chain library to yield a highly diverse fully human Fab phage display library.
Hyperimmune scFv Library
HCDR3 sequence diversities were combinatorially assembled and incorporated into the humanized VH3-23 framework to construct the heavy chain library, which was then paired with a highly diverse kappa VK1-39 light chain library. This combination yields a highly functional antibody library of ~1010 size. Originally offered as a Fab library, the library is now also available in the more compact scFv format.
VHH hShuffle Hyperimmune Library
Offers high diversity human CDR3 with natural llama CDR1/2 sequences in a humanized DP-47 VHH framework. More than two million HCDR3 sequences were gathered from a database of human naïve and memory B-cell receptor sequences and incorporated into the VHH hShuffle library
Hyperimmune Common Light Chain Library
For the common light chain hyperimmune library, trastuzumab light chain is cloned in place of the VK1-39 light chain library.
Learn more about developing diverse synthetic fully human antibody libraries for antiviral therapeutic development.
The Twist Hyperimmune Library synthetically mimics the human in vivo antibody repertoire, providing optimal diversity for antibody development against any target.
This naïve library is the original design of the Twist Hyperimmune Library series, which also includes the VHH hShuffle Hyperimmune (HI) and Common Light Chain Hyperimmune libraries.
Hyperimmune Fab Library
A highly diverse kappa VK1-39 light chain library was paired with the VH3-23 heavy chain library to yield a highly diverse fully human Fab phage display library.
Hyperimmune scFv Library
HCDR3 sequence diversities were combinatorially assembled and incorporated into the humanized VH3-23 framework to construct the heavy chain library, which was then paired with a highly diverse kappa VK1-39 light chain library. This combination yields a highly functional antibody library of ~1010 size. Originally offered as a Fab library, the library is now also available in the more compact scFv format.
VHH hShuffle Hyperimmune Library
Offers high diversity human CDR3 with natural llama CDR1/2 sequences in a humanized DP-47 VHH framework. More than two million HCDR3 sequences were gathered from a database of human naïve and memory B-cell receptor sequences and incorporated into the VHH hShuffle library
Hyperimmune Common Light Chain Library
For the common light chain hyperimmune library, trastuzumab light chain is cloned in place of the VK1-39 light chain library.
Learn more about developing diverse synthetic fully human antibody libraries for antiviral therapeutic development.
Twist’s Hyperimmune Library was panned against the SARS-CoV-2 S1 Spike antigen. A large number of unique clones including TB182-3, -4, and -7, were identified possessing a range of binding affinities. Their activities were demonstrated in competition and functional studies.
This diverse Hyperimmune Library is screened against target antigens. Functional antibodies can be obtained in 8+ weeks.
Twist’s Hyperimmune Library has been effective at uncovering SARS-CoV-2 S1 Protein virus leads as well as broad CD3e
discovery effort.
TB182-3 and TB-182-4 Show Potent Inhibition of S1 Binding to ACE2-expressing VERO E6 cells.
Twist’s Hyperimmune Library was panned against the SARS-CoV-2 S1 Spike antigen. A large number of unique clones including TB182-3, -4, and -7, were identified possessing a range of binding affinities. Their activities were demonstrated in competition and functional studies.
This diverse Hyperimmune Library is screened against target antigens. Functional antibodies can be obtained in 8+ weeks.
Twist’s Hyperimmune Library has been effective at uncovering SARS-CoV-2 S1 Protein virus leads as well as broad CD3e
discovery effort.
TB182-3 and TB-182-4 Show Potent Inhibition of S1 Binding to ACE2-expressing VERO E6 cells.
A synthetic phage antibody library was derived from public databases of naïve and memory B-cell receptor sequences from three human donors. More than two million HCDR3 sequences were gathered and constructed with Twist’s DNA synthesis capabilities.
- Humanized DP-47 framework
- > 2 million human CDR3 sequences
- Highly functional Fab library
- Final library diversity = 1 x 1010
A synthetic phage antibody library was derived from public databases of naïve and memory B-cell receptor sequences from three human donors. More than two million HCDR3 sequences were gathered and constructed with Twist’s DNA synthesis capabilities.
- Humanized DP-47 framework
- > 2 million human CDR3 sequences
- Highly functional Fab library
- Final library diversity = 1 x 1010
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