Twist’s Multiplexed Gene Fragments (MGF) offer a unique pooled format for up to hundreds of thousands of double-stranded DNA gene fragments, between 301 and 500 base pairs in length. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.