I am receiving my samples in media as Supernatants, how do I purify my antibodies?
You can use a Protein A or G solid phase to purify, which binds to the FC region of the antibodies, depending on species and isotype of IgG. Alternatively, you can use immobilized antigen (full length or short peptide antigen) to purify the antibodies. The method will depend on the downstream application and specificity of the antibody.
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