Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers. Alternatively, eliminate the PCR step altogether with Twist Full Length UDI Adapters.
Twist Full Length UDI Adapters
Twist's latest NGS adapter sets are full length unique dual index adapters (10 bp in length) that enable PCR-free whole genome sequencing (WGS).
With 1,536 adapters available, users have the ability to multiplex and to maximize the efficiency of utilization of sequencing reads in a sequencing run.
The adapter sets are functional validated to ensure performance and reliability in all NGS applications. The 96-well plate configuration is designed to be automation-friendly, which can reduce hands-on-time and human error.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist Universal Adapter System (10 bp)
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist HT Universal Adapter System (12 bp)
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers. Alternatively, eliminate the PCR step altogether with Twist Full Length UDI Adapters.
Twist Full Length UDI Adapters
Twist's latest NGS adapter sets are full length unique dual index adapters (10 bp in length) that enable PCR-free whole genome sequencing (WGS).
With 1,536 adapters available, users have the ability to multiplex and to maximize the efficiency of utilization of sequencing reads in a sequencing run.
The adapter sets are functional validated to ensure performance and reliability in all NGS applications. The 96-well plate configuration is designed to be automation-friendly, which can reduce hands-on-time and human error.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist Universal Adapter System (10 bp)
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist HT Universal Adapter System (12 bp)
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Alternatively, ligation using Twist Full Length UDI Adapters allows for the preparation of ready-to-sequence libraries without the need for amplification.
![NGS Reagents and Kits Universal Adapters Workflow](/sites/default/files/2023-10/102623-web-library-workflow-vertical.png)
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Alternatively, ligation using Twist Full Length UDI Adapters allows for the preparation of ready-to-sequence libraries without the need for amplification.
![NGS Reagents and Kits Universal Adapters Workflow](/sites/default/files/2023-10/102623-web-library-workflow-vertical.png)
107376
Twist Full Length UDI Adapters, Plate 1107377
Twist Full Length UDI Adapters, Plate 2107378
Twist Full Length UDI Adapters, Plate 3107379
Twist Full Length UDI Adapters, Plate 4107383
Twist Full Length UDI Adapters, Plate 5107384
Twist Full Length UDI Adapters, Plate 6107385
Twist Full Length UDI Adapters, Plate 7107386
Twist Full Length UDI Adapters, Plate 8107387
Twist Full Length UDI Adapters, Plate 9107388
Twist Full Length UDI Adapters, Plate 10107389
Twist Full Length UDI Adapters, Plate 11107390
Twist Full Length UDI Adapters, Plate 12107391
Twist Full Length UDI Adapters, Plate 13107392
Twist Full Length UDI Adapters, Plate 14107393
Twist Full Length UDI Adapters, Plate 15107394
Twist Full Length UDI Adapters, Plate 16107381
Twist Full Length UDI Adapters, 384 samples- Set 1107469
Twist Full Length UDI Adapters, 384 samples- Set 2107470
Twist Full Length UDI Adapters, 384 samples- Set 3107471
Twist Full Length UDI Adapters, 384 samples- Set 4106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate DThe Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
107376
Twist Full Length UDI Adapters, Plate 1107377
Twist Full Length UDI Adapters, Plate 2107378
Twist Full Length UDI Adapters, Plate 3107379
Twist Full Length UDI Adapters, Plate 4107383
Twist Full Length UDI Adapters, Plate 5107384
Twist Full Length UDI Adapters, Plate 6107385
Twist Full Length UDI Adapters, Plate 7107386
Twist Full Length UDI Adapters, Plate 8107387
Twist Full Length UDI Adapters, Plate 9107388
Twist Full Length UDI Adapters, Plate 10107389
Twist Full Length UDI Adapters, Plate 11107390
Twist Full Length UDI Adapters, Plate 12107391
Twist Full Length UDI Adapters, Plate 13107392
Twist Full Length UDI Adapters, Plate 14107393
Twist Full Length UDI Adapters, Plate 15107394
Twist Full Length UDI Adapters, Plate 16107381
Twist Full Length UDI Adapters, 384 samples- Set 1107469
Twist Full Length UDI Adapters, 384 samples- Set 2107470
Twist Full Length UDI Adapters, 384 samples- Set 3107471
Twist Full Length UDI Adapters, 384 samples- Set 4106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate DThe Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Safety Data Sheet for 101308
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Safety Data Sheet for 101307
Safety Data Sheet
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Safety Data Sheet for 104439
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Safety Data Sheet for 101308
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Safety Data Sheet for 101307
Safety Data Sheet
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Safety Data Sheet for 104439
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Poster
UDI Primers and Universal Blockers Enable Multiplexed Target Enrichments (ASHG 2019)