Site Saturation Variant Libraries

Generate 99% of Desired Variants

Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.

Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.

KEY BENEFITS

Precisely Crafted Variant Libraries

Complete control over codon usage (all 64 codons available)

High uniformity of variant representation at every site

No unwanted codons or premature stop codons

Verified Quality

Rigorous quality control

Verified uniformity of variant representation by NGS

Representation of each site normalized by mass

Flexibility

One position per well in a 96 well plate or all positions pooled in a single tube

Screen 1 to 20 different amino acids at each position

Start Designing Your Library Now - A team of experts is waiting to help you design your perfect library for your specific needs. Order and Design here.

KRAS through large-scale saturation mutagenesis screens

Learn more about how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.

Learn more

Experience base by base precision

Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.

Watch the Webinar

Did you know that tumor evolution is a significant problem for drug developers?

Learn how Twist is helping drug developers characterize and catalog mutations in KRAS through large-scale saturation mutagenesis screens.

WATCH THE VIDEO

	Error Prone PCR	Degenerate (NNK/NNS)	Twist Site Saturation Variant Libraries
Eliminates sequence bias	No	No	Yes
Number of codons available	Unknown	32	All 64
Prevents undesirable motifs	No	No	Yes
Allows codon optimization	No	No	Yes
Avoids stop codons	No	Yes	Yes

A New Way of Designing Your Libraries

The New Library Design (DLD) Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

Start Design Now

Design Companion

Intuitive, interactive portal to easily upload your library sequence file

Identify potential errors in sequence with instant feedback enabling library validation on the fly

Save drafts for future reference

Real-time Optimization

Errors are flagged for review, letting you quickly resolve issues without delays

Our library design experts are still ready to assist you with any questions

With our intuitive tool and expert support we can help you find the fastest path to a validated, high-quality library

Faster Process

Real-time, interactive library design interface supports optimizing your library design and cost efficiently

Save drafts for future reference

Automatically generates a Statement of Work (SOW) for your order, expediting the entire ordering process

Expert Support Still at Your Fingertips

If you have any questions, please feel free to email us at library@twistbioscience.com

Take a peek at our Library Design Tool

A New Way of Designing Your Libraries at Twist

Watch Step by Step how to optimize your sequences

START DESIGN NOW

Let’s get Started

The New Library Design Tool is your all in one solution to streamline, optimize, and expedite the DNA Library design and ordering process.

Step 1

Log into your account. If you don’t have one, it only takes a few minutes to set it up!

Step 2

Start to design your library in the new interactive libraries design tool which has a step by step “how to” wizard. With this option you can optimize your library in real time with validations on the fly and real-time pricing and receive an SOW at the end of the submission process.

Step 3

Once the quote is accepted by the customer, the Libraries Team will send the project to the production team

Start Design Now

Note:

The Libraries Design Tool is not currently available in the APAC region. Please check with your local support team on availability date.

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