Ancestral scFv Library

The Twist Ancestral scFv Library leverages information from 22,426 existing human, monkey, and alpaca antibodies. The library excludes potential development liability motifs while selecting for optimal complementarity determining region (CDR) sequences and lengths that generate the diversity of this antibody set. The heavy chain library permutates CDR sequences from 100 unique CDR1s, 100 unique CDR2s, and 845 unique CDR3s within the human IGHV3-23 framework. The light chain library assembles diverse combinations of CDRs from 80 unique CDR1s, 80 unique CDR2s, and 400 unique CDR3s within the human IGKVK1-39 framework. When combined, the assembled heavy and light chain libraries yield a fully human scFv library with a diversity of 1 x 10⁹.

Go from panning to functional assays in 10-12 weeks. The process starts with phage screening the diverse Twist Ancestral scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Ancestral scFv library to initiate your own in-house discovery projects. To learn more, get in touch at biopharma@twistbioscience.com.

Kinetics with directly coupled anti-S1 antibodies via surface plasmon resonance identifies antibodies like TB212-12 and TB212-56 with high binding affinity for S1 and S trimer.

Flow titration demonstrates that TB212-12 and TB212-56 show inhibition of S1 binding to ACE2-expressing VERO E6 cell