Select your Library prep
EF1.0 + UDI
Enzymatic Fragmentation and Twist Universal Adapter System
EF2.0 + UDI
Enzymatic Fragmentation 2.0 and Twist Universal Adapter System
Mech LP + UDI
Mechanical Fragmentation Library Prep and Twist Universal Adapter System
Mech LP + UMI
Mechanical Fragmentation Library Prep and Twist Universal Adapter System
NEB EM-Seq Library Prep
NEBNext Enzymatic Methyl-seq Library Preparation Protocol
EF1.0 + UDI
Enzymatic Fragmentation and Twist Universal Adapter System
| Reagent | Single Reaction (uL) | Total Volume (uL) |
|---|---|---|
| Enzymatic Fragmentation Master Mix | ||
| Water (chilled) | 25 | |
| 10x Fragmentation Buffer | 5 | |
| 5x Fragmentation Enzyme | 10 | |
| Reaction Mix | 40 | |
| Sample | 10 | |
| Total (Frag/AT rxn) | 50 | |
| Ligation Master Mix | ||
| Water (chilled) | 15 | |
| DNA Ligation Buffer | 20 | |
| DNA Ligation Mix | 10 | |
| Reaction Mix | 45 | |
| Twist Universal Adapter | 5 | |
| Frag/AT rxn | 50 | |
| Total | 100 | |
| Twist UDI Indexing PCR | ||
| Equinox Library Amp Mix (2x) | 25 | |
| Twist UDI Primer | 10 | |
| Reaction Mix | 35 | |
| Sample | 15 | |
| Total | 50 | |
How to
This tool has been designed to calculate master mix volumes for use during Enyzmatic ...
This tool has been designed to calculate master mix volumes for use during Enyzmatic Fragmentation Library prep Kit 1 with UDI adapters. To use, enter the desired number of reactions in the highlighted field below to calculate volumes necessary for each step of the library preparation.
IMPORTANT: This tool automatically incorporates 10% dead volume to compensate for transfer loss
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