Twist UMI Adapter System

Overview

The Twist UMI Adapter system offers a sensitive method for identifying low frequency somatic variants from cell free DNA (cfDNA). Libraries prepared with the UMI adapter system can be used for building consensus reads during analysis to increase variant detection sensitivity and to reduce PCR error contribution.

Engineered for optimal performance with heterogenous sample types, the system is available in either 16 or 96 sample formats. It contains:

Twist Unique Molecular Identifier (UMI) Adapters - matched unique molecular indices (two for each molecule) that are compatible with 'T-A' overhang workflows.
Twist 10 bp Unique Dual-Indexed (UDI) Primers - Designed with unique dual indices for each mixed primer pair and provided in a 96-well plate format suitable for automation applications.

KEY BENEFITS

High Sensitivity

Matched adapters enable superior identification of PCR errors

Reduces false positives of low frequency variants for cancer research and discovery

Compatible with Twist’s NGS Solutions

Easy integration with Twist Mechanical Fragmentation library prep workflow

Tested for performance with Twist’s target enrichment panels

Appropriate for somatic applications

Accepts gDNA or cfDNA input

Scalable formats for 16 or 96 samples

Want to Learn More?

Learn how to properly implement UMIs in your sequencing workflow

Download

The Twist UMI Adapter system offers a sensitive method for identifying low frequency somatic variants from cell free DNA (cfDNA). Libraries prepared with the UMI adapter system can be used for building consensus reads during analysis to increase variant detection sensitivity and to reduce PCR error contribution.

Engineered for optimal performance with heterogenous sample types, the system is available in either 16 or 96 sample formats. It contains:

Twist Unique Molecular Identifier (UMI) Adapters - matched unique molecular indices (two for each molecule) that are compatible with 'T-A' overhang workflows.
Twist 10 bp Unique Dual-Indexed (UDI) Primers - Designed with unique dual indices for each mixed primer pair and provided in a 96-well plate format suitable for automation applications.

KEY BENEFITS

High Sensitivity

Matched adapters enable superior identification of PCR errors

Reduces false positives of low frequency variants for cancer research and discovery

Compatible with Twist’s NGS Solutions

Easy integration with Twist Mechanical Fragmentation library prep workflow

Tested for performance with Twist’s target enrichment panels

Appropriate for somatic applications

Accepts gDNA or cfDNA input

Scalable formats for 16 or 96 samples

Want to Learn More?

Learn how to properly implement UMIs in your sequencing workflow

Download

DATA

Compatible with Twist NGS workflows

Twist’s comprehensive NGS toolkit has what you need to take your low input sample from DNA to data. The Twist UMI adapter system integrates seamlessly into existing library preparation workflows, either as a drop-in replacement for Twist Universal adapters or as an additional step.

Greater sensitivity in variant detection

Twist’s UMI adapter system offers a tool for consensus building that removes errors from PCR duplicates.

Compatible with Twist NGS workflows

Twist’s comprehensive NGS toolkit has what you need to take your low input sample from DNA to data. The Twist UMI adapter system integrates seamlessly into existing library preparation workflows, either as a drop-in replacement for Twist Universal adapters or as an additional step.

Greater sensitivity in variant detection

Twist’s UMI adapter system offers a tool for consensus building that removes errors from PCR duplicates.

Ordering & Specs

Product SKUs

105040

Twist UMI Adapter System TruSeq Compatible, 16 Samples

105041

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate A

105042

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate B

105043

Twist UMI Adapter System TruSeq Compatible, 96 Samples Plate C