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protocols.io,
Dec 2023
DOI: 10.17504/protocols.io.bp2l6xy15lqe/v1
TF and epigenetic modifier CRISPRi/a screens in human T cells v1
R. McCutcheon, Sean ;
M. Swartz, Adam ;
C. Brown, Michael ;
Barrera, Alejandro ;
McRoberts Amador, Christian ;
Humayun, Lucas ;
A. ter Weele, Maria ;
R Daniel, Andrea ;
E. Reddy, Timothy ;
S. Allen, Andrew ;
K. Nair, Smita ;
J. Antonia, Scott ;
A. Gersbach, Charles
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ABSTRACT
This protocol describes methods for CRISPR interference or activation screens identifying transcriptional and epigenetic regulators of human CD8+ T cell state.
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