Single cell CRISPRi screen to identify mechanoenhancer gene targets v1

protocols.io, Aug 2024

DOI: 10.17504/protocols.io.kqdg3xd7zg25/v1

Single cell CRISPRi screen to identify mechanoenhancer gene targets v1

D. Cosgrove, Brian ; Key Taylor, Carson ; Barrera, Alejandro ; R Daniel, Andrea ; E. Crawford, Gregory ; A. Gersbach, Charles

PRODUCTS USED

Variant Libraries

Read Full Article

ABSTRACT

This protocols describes methods for a single cell CRISPR interference screen to identify gene targets regulated by mechanoenhancers that drive cellular growth and migration.

Read Full Article

PRODUCTS USED

Variant Libraries

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Single cell CRISPRi screen to identify mechanoenhancer gene targets v1

PRODUCTS USED

ABSTRACT

PRODUCTS USED

Related Publications

Genetic hearing loss in children-Genetic variation and parental experiences of genetic diagnostics

Comprehensive characterization of early-stage Non-Small Cell Lung cancers with multi-modal data integration

Generating long deletions across the genome with pooled paired prime editing screens