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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Low bias, high fidelity amplification built for NGS Workflows.
Engineered for NGS library amplification, Twist TrueAmp Polymerase delivers uniform coverage across GC extremes, high sensitivity from low inputs, and automation ready hot start performance.
Unbiased, High Fidelity Results
Amplify uniformly across extreme 5–95% GC regions to maintain coverage of difficult targets. Improved fidelity and reduced homopolymer slippage deliver cleaner data and more confident calls with fewer repeats.
Reliable Performance from Challenging Samples
Generate consistent, high-yield libraries from complex samples—even at ≤100 pg input—so you recover more usable data and keep your workflows moving forward.
Simplified, Scalable Workflows
An aptamer enabled hot start enables room temperature stability for automation, while a single-tube 2× mastermix simplifies setup and reduces hands-on time; helping you scale workflows with fewer errors and faster turnaround.
Product data
TrueAmp sustains coverage where others drop out, improving sequencing economics and preserving difficult targets.
Figure 1 GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.
With improved efficiency, TrueAmp can hit yield targets in fewer cycles, limiting cycle-induced artifacts. It remains robust for low-yield libraries typical of post-capture PCR.
Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.
An engineered proofreading polymerase reduces C→T misincorporations often caused by cytosine deamination and limits slippage across long homopolymers—critical for sensitive variant detection.
Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.
Magnetic beads are widely used for purification and size selection but can inhibit PCR and lower yields.
Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.
Video
Low-bias amplification across GC extremes
TrueAmp sustains coverage where others drop out, improving sequencing economics and preserving difficult targets.
Figure 1 GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.
Efficient & robust yields at low input
With improved efficiency, TrueAmp can hit yield targets in fewer cycles, limiting cycle-induced artifacts. It remains robust for low-yield libraries typical of post-capture PCR.
Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.
High fidelity & reduced artifacts
An engineered proofreading polymerase reduces C→T misincorporations often caused by cytosine deamination and limits slippage across long homopolymers—critical for sensitive variant detection.
Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.
Bead Tolerance for Reliable PCR
Magnetic beads are widely used for purification and size selection but can inhibit PCR and lower yields.
Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.
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Low-bias amplification across GC extremes
TrueAmp sustains coverage where others drop out, improving sequencing economics and preserving difficult targets.
Efficient & robust yields at low input
With improved efficiency, TrueAmp can hit yield targets in fewer cycles, limiting cycle-induced artifacts. It remains robust for low-yield libraries typical of post-capture PCR.
High fidelity & reduced artifacts
An engineered proofreading polymerase reduces C→T misincorporations often caused by cytosine deamination and limits slippage across long homopolymers—critical for sensitive variant detection.
Bead Tolerance for Reliable PCR
Magnetic beads are widely used for purification and size selection but can inhibit PCR and lower yields.
Highlighted resources
Case study
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Ebook
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Ebook
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Poster
GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits
Ebook
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