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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Engineered Performance for Reliable, Scalable cfDNA Workflows
Generate high quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Preparation Kit helps you uncover critical insights with confidence and efficiency.
Maximize Rare Variant Detection
High conversion rate reliably detects rare variants (≤0.1% VAF). High coverage libraries compatible with duplex sequencing workflows supported by the Twist UMI Adapter System.
Streamline Workflow of Precious Samples
Robust performance, even with low (<1ng) sample input Sequence-ready libraries in 2 hours.
Amplification without Bias
Delivers consistent coverage across 5%–95% GC, preserving visibility of challenging genomic regions and difficult-to-capture targets. Consistent high yields from complex libraries —even at ≤100 pg DNA input. Call variants with confidence via improved fidelity and reduced homopolymer slippage
Product data
High Fidelity Amplification with PCR Free–Like Error Rates cfDNA libraries prepared with Twist TrueAmp Polymerase show substitution error rates that are effectively equivalent to PCR-free cfDNA libraries across all measured base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Preparation Kit was used to generate libraries with Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water and 1 µL was quantified with QubitTM dsDNA Broad Range Kit.
Figure 2. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Twist cfDNA Library Prep and Hyb Mix Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 SNPs. Missed variants are sites with <1 variant family over total detectable sites.
Figure 3. Twist cfDNA Library Preparation and Hyb Mix Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Twist cfDNA Library Prep and Hyb Mix Kit delivers higher library complexity with or without UMI deduplication. Single plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized TE workflows.
Figure 4. Twist cfDNA Library Preparation and Hyb Mix Kit delivers higher complexity with and without UMI deduplication.
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 μL of water and 1 μL was quantified with QubitTM dsDNA Broad Range Kit.
Figure 5. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Video
High Fidelity Amplification with PCR
High Fidelity Amplification with PCR Free–Like Error Rates cfDNA libraries prepared with Twist TrueAmp Polymerase show substitution error rates that are effectively equivalent to PCR-free cfDNA libraries across all measured base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Preparation Kit was used to generate libraries with Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Higher Conversion Rate = Higher Yield
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water and 1 µL was quantified with QubitTM dsDNA Broad Range Kit.
Figure 2. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Higher Sensitivity & Higher Accuracy
Twist cfDNA Library Prep and Hyb Mix Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 SNPs. Missed variants are sites with <1 variant family over total detectable sites.
Figure 3. Twist cfDNA Library Preparation and Hyb Mix Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
mproved Duplexing = Higher Complexity
Twist cfDNA Library Prep and Hyb Mix Kit delivers higher library complexity with or without UMI deduplication. Single plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized TE workflows.
Figure 4. Twist cfDNA Library Preparation and Hyb Mix Kit delivers higher complexity with and without UMI deduplication.
Low Input Proven with native cfDNA
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 μL of water and 1 μL was quantified with QubitTM dsDNA Broad Range Kit.
Figure 5. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
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High Fidelity Amplification with PCR
High Fidelity Amplification with PCR Free–Like Error Rates cfDNA libraries prepared with Twist TrueAmp Polymerase show substitution error rates that are effectively equivalent to PCR-free cfDNA libraries across all measured base changes.
Higher Conversion Rate = Higher Yield
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water and 1 µL was quantified with QubitTM dsDNA Broad Range Kit.
Higher Sensitivity & Higher Accuracy
Twist cfDNA Library Prep and Hyb Mix Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 SNPs. Missed variants are sites with <1 variant family over total detectable sites.
mproved Duplexing = Higher Complexity
Twist cfDNA Library Prep and Hyb Mix Kit delivers higher library complexity with or without UMI deduplication. Single plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized TE workflows.
Low Input Proven with native cfDNA
Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 μL of water and 1 μL was quantified with QubitTM dsDNA Broad Range Kit.
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