What does a leader (signal peptide) sequence do and what leader sequence do we use in our pTwist vectors?

Question

Accepted Answer

A leader or secretory signal sequence acts as a peptide signal bound by cellular machinery early in the translation process. This allows for the correct localization of the translation complex to the endoplasmic reticulum where the antibody chains can enter into the secretory pathway for protein assembly and secretion from the cell. The leader sequence is cleaved during the protein assembly process and is not a part of the final protein. Twist recommends the following leader sequence for both heavy and light chains when using our pTwist IgG expression vectors as these sequences have been tested and validated with our pTwist vectors in both Expi293™ and ExpiCHO™ cell lines:VH: MRAWIFFLLCLAGRALAVL: MRAWIFFLLCLAGRALA

基因

寡核苷酸池

NGS 靶向富集解决方案

基因突变文库

合成质控品

抗体发现

药物研发工具

传染病检测工具

概述

抗体

常规问题

抗体

前导（信号肽）序列有什么作用？pTwist 载体中使用的是什么前导序列？

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