概述

Nanobodies: Small but Mighty

The unique properties of VHHs (single-domain antibodies, scAbs, or nanobodies) overcome these challenges, making them a promising antibody format for therapeutics, reagents, or diagnostics. Regardless of your application, we offer comprehensive capabilities leveraging in vivo, in vitro, and in silico workflows to provide you with VHH antibodies with desired specificities and functional properties such as affinities in single-digit and subnanomolar ranges.

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Flexible, Customizable Workflows for VHHs


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In Vivo Workflows: Superior Targeting of Cell Surface
Proteins with Rapid Turnaround Times

Screen with speed

Twist has developed an optimized in vivo discovery workflow based on immunized camelids tailored for cell surface targets. We leverage an industry-leading, Beacon-based, single B cell screening platform, enabling the rapid identification of binders with required activities and/or cross-reactivity in 1 day.

Never miss a hit

We screen IgG2/3-secreting alpaca B cells for specificity and function using a high-resolution screening cascade that includes up to four on-cell assays before sequence recovery. Integrated cell line development services enhance our streamlined workflow, ensuring efficient and effective assays and outcomes. 

免版税

Twist also offers royalty-free, flexible business models to accommodate different partners' needs, including fee-for-service and equity-for-service structures.

Nanobodies: Small but Mighty

The unique properties of VHHs (single-domain antibodies, scAbs, or nanobodies) overcome these challenges, making them a promising antibody format for therapeutics, reagents, or diagnostics. Regardless of your application, we offer comprehensive capabilities leveraging in vivo, in vitro, and in silico workflows to provide you with VHH antibodies with desired specificities and functional properties such as affinities in single-digit and subnanomolar ranges.

曲线图

Flexible, Customizable Workflows for VHHs


曲线图

In Vivo Workflows: Superior Targeting of Cell Surface
Proteins with Rapid Turnaround Times

Screen with speed

Twist has developed an optimized in vivo discovery workflow based on immunized camelids tailored for cell surface targets. We leverage an industry-leading, Beacon-based, single B cell screening platform, enabling the rapid identification of binders with required activities and/or cross-reactivity in 1 day.

Never miss a hit

We screen IgG2/3-secreting alpaca B cells for specificity and function using a high-resolution screening cascade that includes up to four on-cell assays before sequence recovery. Integrated cell line development services enhance our streamlined workflow, ensuring efficient and effective assays and outcomes. 

免版税

Twist also offers royalty-free, flexible business models to accommodate different partners' needs, including fee-for-service and equity-for-service structures.

优势

graphtimeline

In Vitro Workflows: Ready-to-Use or Custom Discovery Libraries

Twist Biosciences’ precision DNA synthesis enables researchers to explore a vast, pre-defined sequence that focuses explicitly on the VHH sequences with the best chance of success.

Novel approach

We use multiple novel VHH sequence incorporation that combine synthetic and natural approaches to maximize diversity, creating high-quality, off-the-shelf VHH libraries for use against any target. 

Unique and proprietary

These proprietary, naïve single-domain libraries are ideal for initiating antibody discovery and development and have been validated against immuno-oncology, CAR-T, GPCR, ion channel, and transporter targets.

Customization when you need it

We can also design and generate highly-diverse custom VHH libraries tailored to your program's needs in as little as 3 to 4 weeks.

 

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Immune Library

Our scientists work closely with you to develop a custom VHH antibody discovery strategy that combines our in vitro and in vivo capabilities. We immunize camelids with the target antigen and isolate peripheral blood lymphocytes. The isolated lymphocytes are then used to construct a unique library of VHH antibody sequences. Our skilled scientists provide a custom library with high diversity and optimal biophysical properties, ensuring specificity and affinity.

VHH Optimization, Expression, and Characterization Under One Roof

graphtimeline

In Vitro Workflows: Ready-to-Use or Custom Discovery Libraries

Twist Biosciences’ precision DNA synthesis enables researchers to explore a vast, pre-defined sequence that focuses explicitly on the VHH sequences with the best chance of success.

Novel approach

We use multiple novel VHH sequence incorporation that combine synthetic and natural approaches to maximize diversity, creating high-quality, off-the-shelf VHH libraries for use against any target. 

Unique and proprietary

These proprietary, naïve single-domain libraries are ideal for initiating antibody discovery and development and have been validated against immuno-oncology, CAR-T, GPCR, ion channel, and transporter targets.

Customization when you need it

We can also design and generate highly-diverse custom VHH libraries tailored to your program's needs in as little as 3 to 4 weeks.

 

graph5

Immune Library

Our scientists work closely with you to develop a custom VHH antibody discovery strategy that combines our in vitro and in vivo capabilities. We immunize camelids with the target antigen and isolate peripheral blood lymphocytes. The isolated lymphocytes are then used to construct a unique library of VHH antibody sequences. Our skilled scientists provide a custom library with high diversity and optimal biophysical properties, ensuring specificity and affinity.

VHH Optimization, Expression, and Characterization Under One Roof

技术资源

抗体工程与优化

  • Rapid, precise creation of custom antibody libraries tailored to enhancing your therapeutic antibody candidates
  • Affinity maturation: Rapid, high-diversity in silico screening to deliver variants with improved affinity
  • Humanization: Service to engineer humanized antibodies, maintaining desired affinity, safety, and efficacy profiles

Express Antibody Production and Sequencing

  • Small- to medium-scale HT VHH-Fc production: CHO (13-18 days), HEK (10-15 days)
  • Bispecific matrixing: Reformatting scFv, Fab, bi- and multi-specifics to design and generate complex molecular formats
  • NGS sequencing: AI/ML support

抗体表征

  • Binding confirmation: ELISA, flow cytometry, Octet
  • Affinity ranking and KD determination: SPR, Octet, flow cytometry
  • Epitope binning: SPR, Octet
  • Functional and bioanalytical: Internalization, cytotoxicity, ligand blocking, endotoxin testing, recruitment or inhibition, cross-reactivity
  • Developability assessment: In silico analysis, CIC, AC-SINS, HIC, BVP-ELISA, aSEC, stability

技术资源

单结构域抗体文库 (VHH)

Case Studies Antibodies

下载产品说明书

技术资源

单结构域抗体文库 (VHH)

Case Studies Antibodies

下载产品说明书

抗体工程与优化

  • Rapid, precise creation of custom antibody libraries tailored to enhancing your therapeutic antibody candidates
  • Affinity maturation: Rapid, high-diversity in silico screening to deliver variants with improved affinity
  • Humanization: Service to engineer humanized antibodies, maintaining desired affinity, safety, and efficacy profiles

Express Antibody Production and Sequencing

  • Small- to medium-scale HT VHH-Fc production: CHO (13-18 days), HEK (10-15 days)
  • Bispecific matrixing: Reformatting scFv, Fab, bi- and multi-specifics to design and generate complex molecular formats
  • NGS sequencing: AI/ML support

抗体表征

  • Binding confirmation: ELISA, flow cytometry, Octet
  • Affinity ranking and KD determination: SPR, Octet, flow cytometry
  • Epitope binning: SPR, Octet
  • Functional and bioanalytical: Internalization, cytotoxicity, ligand blocking, endotoxin testing, recruitment or inhibition, cross-reactivity
  • Developability assessment: In silico analysis, CIC, AC-SINS, HIC, BVP-ELISA, aSEC, stability

联系我们

我们的抗体专家随时待命,并希望听到更多关于您的发现需求的信息。欢迎挑战性目标!

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