Twist RNA Sequencing Workflow Solutions

Twist’s RNA sequencing workflows offer a complete NGS solution that produces uniform libraries for RNA sequencing. Twist offers targeted and whole transcriptome workflows that reduce time at the bench and integrate with our current set of NGS reagents, including our target enrichment kits. Each workflow delivers high quality libraries ready for sequencing from a wide range of inputs and sample types, including translation research samples.

Twist Custom RNA Panels can be designed and built to cover a wide range of panel sizes and transcript target regions with the exceptional and consistent performance. Utilizing our deep design experience, Twist has developed exon-aware design algorithms to target individual exons while not preferentially selecting any one exon-exon junction site. We’ve applied this design approach to the Twist RNA Exome which makes it a good tool for transcriptome analysis and fusion detection.

The end-to-end workflows achieve this by combining streamlined and effective library preparation kits, high quality unique dual indices (UDI), unique molecular identifiers, optimized target enrichment, and proprietary exon-aware panel design algorithm.

Targeted RNA Sequencing

• ​​Create custom panels to sequence only the RNA transcripts that matter 
• Combine Twist RNA Library Prep, Twist Hybridization and RNA target enrichment panels
• Enrich sensitivity for key targets even in low quality, degraded RNA from FFPE

RNA Exome & Targeted Sequencing

• ​​Exon-aware probe design for protein coding regions and unique splice sites
• Fewer reads per sample for more samples
• Targets 19708 genes and 63215 isoforms 
• Greater than 1.8 fold enrichment over whole transcriptome

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Whole Transcriptome Sequencing

• Measure expression levels of mRNA, lncRNA and other RNA species
• Prepare libraries from Total RNA extracted from fresh and FFPE samples
• Combine our Twist RNA Library Prep with Twist rRNA & Globin Depletion kit
• Perform RNA Library Preparation in less than 5 hours

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RNA Fusion Detection

• Enrich for known and novel fusions with the Twist Alliance CeGaT Fusion Panel
• Expert content design through collaboration with CeGaT
• Optimized complete workflow with Twist RNA Library Prep kit for sequencing ready library
• Leverage Twist core capture enrichment technology to maximize efficiency

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RNA Exome

The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.

More Targets, Fewer Reads

Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep.

RNA Fusion

Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.

This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.

The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*

Panel Design

• 160 fusion associated genes with 66 genes in the breakpoint design
• 7394 Probes
• Covers a variety of different cancer types

* Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB is ISO-13485 certified.