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PCR-Free WGS Library Preparation Kit
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Low-bias amplification across GC extremes

TrueAmp sustains coverage where others drop out, improving sequencing economics and preserving difficult targets.

Efficient & robust yields at low input

With improved efficiency, TrueAmp can hit yield targets in fewer cycles, limiting cycle-induced artifacts. It remains robust for low-yield libraries typical of post-capture PCR.

High fidelity & reduced artifacts

An engineered proofreading polymerase reduces C→T misincorporations often caused by cytosine deamination and limits slippage across long homopolymers—critical for sensitive variant detection.

Bead Tolerance for Reliable PCR

Magnetic beads are widely used for purification and size selection but can inhibit PCR and lower yields.

Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.

Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.

Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.

Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.

Low-bias amplification across GC extremes

TrueAmp sustains coverage where others drop out, improving sequencing economics and preserving difficult targets.

Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.

Efficient & robust yields at low input

With improved efficiency, TrueAmp can hit yield targets in fewer cycles, limiting cycle-induced artifacts. It remains robust for low-yield libraries typical of post-capture PCR.

Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.

High fidelity & reduced artifacts

An engineered proofreading polymerase reduces C→T misincorporations often caused by cytosine deamination and limits slippage across long homopolymers—critical for sensitive variant detection.

Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.

Bead Tolerance for Reliable PCR

Magnetic beads are widely used for purification and size selection but can inhibit PCR and lower yields.

Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.

Engineered amplification that preserves library complexity

Many PCR enzymes are optimized for single-amplicon tasks (qPCR, cloning) rather than complex NGS libraries.

TrueAmp changes the approach
An engineered proofreading, high-processivity DNA polymerase that delivers best-in-class GC uniformity, yield, and fidelity for library amplification.

The result
Fewer biases toward short, GC-neutral molecules and more usable data per run.

cfDNA Workflow Kit

 

Key features include

  • check Single-tube 2x mastermix simplifies setup and reduces handling errors
  • Compatible with pre-capture and post-capture amplification
  • Designed for same-day or overnight hybridization workflows alongside Twist TE kits
  • Validated with Twist library prep & enrichment reagents to maintain end-to-end performance consistency

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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080