Genes
NGS
Antibodies
IVDR Solutions
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Analyze your gene sequences for manufacturability
Get immediate feedback on potential errors or complexities, and know in seconds which synthesis solutions are optimal for your designs.
Don't have your sequences? Use our price estimator 
Complex features we support
Our Gene Synthesis platform helps you make difficult sequences in predictable timescales. See which complex features we can support below.
Short tandem repeats up to 100 bp
Regions of 3-9 bp that repeat multiple times, with up to 100% homology between repeats.
Long repeats up to 200 bp
Regions ≥ 10 bp with up to 100% homology that repeat more than once within a single sequence.
Inverted repeats (hairpin) up to 100 bp
Repeats complementary to each other, with up to 100% homology between repeats.
Localized GC content between 25-75%
GC content within a 50 bp window is between 25-75%.
Homopolymers GC content up to 30 bp
Runs of an identical base.
Average GC content between 10-90%
Average GC content across the whole sequence.
Gene synthesis offerings
The best way to get your DNA
Clonal Genes
For researchers that need reliable, sequence-perfect DNA they can drop into downstream workflows
- Length: 0.3 kb – 7.0 kb
- Scale: 1 µg – 4 µg | 4 µg – 10 µg | 10 µg – 100 µg | 100 µg – 1 mg†
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Price (by complexity level):
0.3 – 1.8 kb: 9¢/bp (Low/Medium) | 12¢/bp (High)‡
1.8 – 3.2 kb: 13¢/bp (Low/Medium) | 18¢/bp (High)
3.2 – 7.0 kb: 18¢/bp (Low/Medium) | 23¢/bp (High)
Cloning: Cloning vector: $35 | Expression vector: $55 | Custom vector: $75 - Turnaround time (in business days): Express: 4 – 7 | Standard: 10 | High complexity: 15
- Customization options: Animal-Origin-Free prep, BSE/TSE certification, Transfection grade preps, Endotoxin free, Target yield, Poly-A QC
- Error rate: 100% sequence perfect, NGS verified
†For high copy vectors. Low/Medium copy vector performance will vary.
‡0.3 – 0.5 kb genes have a per-sequence price which includes cloning and synthesis.
Gene Fragments
For researchers that need affordable DNA delivered as fast as possible
- Length: 0.3 kb – 5.0 kb
- Scale: 100 ng – 1 µg
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Price:
0.3 – 0.5 kb: $35/sequence
0.5 – 1.8 kb: 7¢/bp
1.8 – 3.2 kb: 8¢/bp
3.2 – 5.0 kb: 9¢/bp - Turnaround time (in business days): 2 – 4
- Error rate: 1:7500
Genes are just one part of the workflow
Customers often pair Twist Genes with the following products
Antibody Discovery Services
Partner with our team of experts to navigate the entire discovery funnel from target to lead.
Oligo Pools
High-diversity, silicon-synthesized oligo pools designed for high-throughput screening and complex library construction.
Libraries
Explore vast sequence space with high-diversity libraries where every variant is base-by-base synthesized and NGS-verified to match your design.
We’re here to help
Twist’s Technical Support team is available to help you tackle your biggest research challenges. If you have any questions about your project, our scientists are here to find the answer.
For Research Use Only.
Not for Diagnostic Procedures.
*This timeframe refers to the typical processing and handling time within our facilities before your order is handed over the shipping carrier. Gene Fragments are shipped in 2-4 business days. Eligible Express Genes ship in 4-7 business days. Average turnaround time for Clonal Genes is 10 business days. Our target completion timeline for clonal genes scored as complex is 15 business days. Turnaround time for Clonal Genes is subject to change based on customizations and sequence complexity. New vector onboarding for Clonal Genes will add additional turnaround time. Orders placed outside of the US will incur additional delivery time.
