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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Unbiased whole genome sequencing
Generate high-quality whole genome libraries without amplification bias. The Twist PCR-Free WGS Library Prep Kit utilizes optimized enzymatic fragmentation and ligation chemistry to preserve native genome representation. Rely on consistent insert sizes and low ligation bias, with the uniform coverage necessary for precise variant characterization across the genome.
Lower AT dropout across the genome
Preserve native genome representation by eliminating PCR amplification. Improve uniform coverage across challenging genomic regions.
Built for scaling up workflows
Optimized performance with Twist's full length UDI adapters support multiplexing up to 1,536 samples in a single run, for maximum usable data.
Maximize conversion. Minimize bias.
Minimized ligation bias and improved conversion efficiency preserve true genome representation and generate more usable sequencing reads from every sample.
Product data
The Twist PCR-Free WGS Library Prep Kit delivers robust library yields and consistent library size even as DNA input is reduced. Engineered ligase chemistry drives efficient, uniform ligation, helping maintain sequencing-ready libraries across input levels.
Figure 1. PCR-free library yield comparison across inputs. Final library concentration (nM) was measured following PCR-free library preparation using the indicated DNA inputs. Twist PCR-Free WGS Library Preparation generated higher library yields than competitor workflows at equivalent inputs and maintained strong performance at lower inputs.
The Twist PCR-Free WGS Library Prep Kit maintains consistent median insert sizes from 300 ng down to 37.5 ng of DNA input. Minimal read overlap across samples ensures efficient sequencing and dependable genome-wide coverage.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
The Twist PCR-Free WGS Library Prep Kit enables precise control of insert size across a broad range, allowing users to tune library preparation to the specific requirements of their sequencing workflows.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.
Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Across DNA inputs, the Twist PCR-Free WGS Library Prep Kit maintains consistent coverage across GC content.
Figure 4. The Twist PCR-Free WGS Library Prep Kit provides more consistent coverage distribution across varying GC content. The kit shows minimal GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in high- and low-GC content regions.
Video
Engineered to preserve library yield at low input
The Twist PCR-Free WGS Library Prep Kit delivers robust library yields and consistent library size even as DNA input is reduced. Engineered ligase chemistry drives efficient, uniform ligation, helping maintain sequencing-ready libraries across input levels.
Figure 1. PCR-free library yield comparison across inputs. Final library concentration (nM) was measured following PCR-free library preparation using the indicated DNA inputs. Twist PCR-Free WGS Library Preparation generated higher library yields than competitor workflows at equivalent inputs and maintained strong performance at lower inputs.
Reliable insert size control across inputs
The Twist PCR-Free WGS Library Prep Kit maintains consistent median insert sizes from 300 ng down to 37.5 ng of DNA input. Minimal read overlap across samples ensures efficient sequencing and dependable genome-wide coverage.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
Controllable fragment lengths for more efficient sequencing
The Twist PCR-Free WGS Library Prep Kit enables precise control of insert size across a broad range, allowing users to tune library preparation to the specific requirements of their sequencing workflows.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit. Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Minimized GC bias for more uniform coverage
Across DNA inputs, the Twist PCR-Free WGS Library Prep Kit maintains consistent coverage across GC content.
Figure 4. The Twist PCR-Free WGS Library Prep Kit provides more consistent coverage distribution across varying GC content. The kit shows minimal GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in high- and low-GC content regions.
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Engineered to preserve library yield at low input
The Twist PCR-Free WGS Library Prep Kit delivers robust library yields and consistent library size even as DNA input is reduced. Engineered ligase chemistry drives efficient, uniform ligation, helping maintain sequencing-ready libraries across input levels.
Reliable insert size control across inputs
The Twist PCR-Free WGS Library Prep Kit maintains consistent median insert sizes from 300 ng down to 37.5 ng of DNA input. Minimal read overlap across samples ensures efficient sequencing and dependable genome-wide coverage.
Controllable fragment lengths for more efficient sequencing
The Twist PCR-Free WGS Library Prep Kit enables precise control of insert size across a broad range, allowing users to tune library preparation to the specific requirements of their sequencing workflows.
Minimized GC bias for more uniform coverage
Across DNA inputs, the Twist PCR-Free WGS Library Prep Kit maintains consistent coverage across GC content.
Built for Whole Genomes: Comprehensive Coverage without Bias
How it works
