Twist for Element, Trinity Freestyle Fast Hyb Workflow

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One Enrichment Workflow. Many Possibilities.

The Twist for Element, Trinity Freestyle Fast Hyb kits provide a user-friendly solution for the enrichment of NGS libraries containing P5 and P7 adapter sequences for sequencing on Element Biosciences platforms.

The traditional hybridization process for targeted panels, like whole exome panels, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.

With the simplified Trinity workflow, you’ll hybridize your DNA library to the target probes of interest, incubate with the panel probes for as little as one hour with our fast hyb reagents, and load it directly on your AVITI sequencer. This drastically reduces manual steps and additional amplification resulting in higher library complexity and lower duplication rates.

Streamlined Workflow, Less Hands-on Time

Go from sample to sequencer in as few as five hours
On-flow cell enrichment removes binding, wash, amplification, and pooling steps
Rapid, 1-hour hybridization time eliminates overnight waits

Flexibility Without Compromise

Seamlessly compatible with any Twist sequencing library using P5 and P7 adapters
Preserves AVITI's exceptional data quality across applications
Dual, independent flow cells deliver adaptable throughput

For research use only. Not for use in any diagnostic or clinical procedures.

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Request additional information about how to streamline your sequencing workflows on Element Bio’s AVITI system.

Rapid Exome Sequencing Using Twist Workflow Kits Reduces Hands-On Time

Comparison of the estimated workflow times for Standard Hybridization v2 with a 16-hour overnight hybridization and the Trinity Freestyle Fast Hyb workflow with a 1-hour hybridization time. Each workflow produces exome-enriched libraries compatible with AVITI sequencing.

Whole Exome and Human Genotyping Panels Have High Sequencing Performance on AVITI

Performance comparison of the native Trinity Fast Hyb workflow and the new Trinity Freestyle workflow paired with Twist Exome 2.0 plus Comprehensive Spike-in. There were 24 exomes sequenced on an AVITI and downsampled to 25 million reads. We also demonstrate the performance of Twist’s FlexPrep UHT paired with Twist Genotyping Human 600k panel for high-throughput genotyping of human samples. There were 120 samples sequenced on an AVITI24, reaching 13 to 28 million reads per sample. Values shown are averages ± standard deviation based on Picard metrics..