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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Reliable, scalable cfDNA workflows
Generate high-quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Prep Kit helps you uncover critical insights with confidence and efficiency.
Maximize rare variant detection
High conversion rate reliably detects rare variants (≤0.1% VAF). Support duplex sequencing workflows with the Twist UMI Adapter System to maximize detection sensitivity.
Streamlined workflow for precious samples
Achieve robust performance, even with low (<1 ng) sample input. Get sequence-ready libraries in 2 hours.
Amplification without bias
Get consistent coverage across 5%–95% GC, preserving challenging genomic regions and difficult-to-capture targets. Get consistent high yields from complex libraries, even at ≤100 pg of DNA input. Call variants with confidence.
Product data
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Figure 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Missed variants are sites with <1 variant family over total detectable sites.
Figure 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
Figure 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
Video
High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Higher conversion rate = higher yield
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Figure 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Higher sensitivity & higher accuracy
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Missed variants are sites with <1 variant family over total detectable sites.
Figure 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Improved duplexing = higher complexity
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
Figure 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
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High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Higher conversion rate = higher yield
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
Higher sensitivity & higher accuracy
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). Missed variants are sites with <1 variant family over total detectable sites.
Improved duplexing = higher complexity
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
Related workflows and applications
