You can use the following methods to clone gene fragments with adapters:

Restriction digestion/Ligation - place restriction sites inside of the Twist adapters for easy cloning

Type IIS or Golden Gate - place restriction sites inside of the Twist adapters for easy cloning TOPO cloning (blunt-end) - the adapter sequences will be cloned in as well

Gateway cloning - add attB sites to the ends of your sequence

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