Gene Fragments are assembled and then checked for size and purity by fragment analysis. They are not sequence-verified. Therefore, Gene Fragments are non-clonal (i.e. they are a mixture of perfect and non-perfect material). If you are planning to clone fragments, it is important that you screen more than one clone. Fragments can range in size from 300 to 1,800 bp in length.

Conversely, Clonal Genes are assembled and then cloned into either a custom vector or one of Twist's own catalog vectors. Clones are then NGS sequence verified to confirm that only the requested sequences are shipped and delivered. Clonal Genes can range in size from 300 to 5,000 bp in length.