Twist will sequence the incoming vector, however, only the region surrounding the insertion site (within 200bp on either side of the insertion site) will be analyzed for discrepancies between what is stated as the Vector Reference Sequence in the received Custom Vector On-boarding form and the QC data.

We will report if the sequence does not match or if large deletions or insertions (>100bp) are observed compared to the Vector Reference Sequence provided by the customer. Please note that we currently do not report on SNPs outside of the 200bp on either side of the insertion site.

If any deviations from the Vector Reference Sequence is found, this could delay the completion time for onboarding.