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Complex features we support

Our Gene Synthesis platform helps you make difficult sequences in predictable timescales. See which complex features we can support below.

Short Tandem Repeats

Regions of 3-9 bp that repeat multiple times, with up to 100% homology between repeats.

Up to 100 bp

We support short tandem repeats spanning up to 100 bp.

Long Repeats

Regions ≥ 10 bp with up to 100% homology that repeat more than once within a single sequence.

Up to 200 bp

We support long repeats up to 200 bp.

Inverted Repeats (Hairpins)

Repeats complementary to each other, with up to 100% homology between repeats.

Up to 100 bp

We support inverted repeats up to 100 bp.

Extreme localized GC content

Local GC content within 50 bp regions.

90% within 50 bp

We support GC content within a 50 bp window between 10% and 90%.

Homopolymers

Runs of an identical base.

Up to 30 bp

We support runs of an identical base up to 30 bp.

High/low average GC content

GC content across the whole sequence.

Between 25 - 75% GC

We support sequences with an average GC content of between 25 and 75%.

Request early access

LOW AND MEDIUM COMPLEXITY DESIGNS −

0.3 - 0.5 kbp

0.5 - 1.8 kbp

1.8 - 3.2 kbp

3.2+ kbp

5+ kbp

Synthesis

Included

$0.09/BP

$0.13/bp

$0.18/bp

Cloning fee (per sequence)

Twist Cloning Vector

$80

$35

Twist Expression Vector

$100

$55

Custom Vector

$120

$75

⚡ Express Service

Varies based on availability. +65% Currently ⓘ

HIGH COMPLEXITY DESIGNS −

0.3 - 0.5 kbp

0.5 - 1.8 kbp

1.8 - 3.2 kbp

3.2 - 5.0 kbp

5+ kbp

Synthesis

Included

$0.12/bp

$0.18/bp

$0.23/bp

Cloning fee for:

Twist Cloning Vector

$95

$35

Twist Expression Vector

$115

$55

Custom Vector

$135

$75

DNA OPTIONS −

Production

1 μg – 4 μg ⚡

4 μg – 10 μg

10 μg - 100 μg

100 μg - 1 mg

DNA Scale

Included

$10

$100

$150

Target Yield

—

Purity Grade

Molecular Grade

Included

—

Transfection Grade (<10EU/μg)

—

$25

Included

Endotoxin Free (<0.1EU/μg)

—

$50

DNA Final State

Normalization

—

$15

Quality Control

Verification Method

Poly(A) Tail Length Verification

$25

Quality Documents

Animal-Origin-Free Certificate

$25

Additional Materials

Glycerol Stock

$10

Product data

Complex DNA. Simple pricing.

With prices starting at 12 cents per base, you get more complex DNA for your budget. Cost-per-base scales with sequence length, not sequence complexity, so pricing stays predictable. And, with early access, you get an immediate quote for your Complex Genes in our ordering portal.

See pricing

US manufacturing

All Gene products are USA-made, shipping globally from our factory in Wilsonville, OR. Here, our scientists use ultra-long oligo synthesis on silicon, automated enzymatic assembly, and long-read sequencing to produce your Complex Gene Synthesis projects.

Reliable turnaround

For Complex Genes, we provide an estimated turnaround time before you order as well as live manufacturing tracking on our portal. We aim to complete all Complex Gene orders within 15 business days. We will contact you immediately should challenges arise.

Support from real scientists

Twist’s Technical and Customer Support teams are available to help you tackle your biggest research challenges. If you ever have a question or issue, click on the chat icon in the bottom-right of our website, and connect with a real scientist in minutes.