Discover New Viral Species
The pandemic emergence of SARS-CoV-2 along with the mpox outbreak highlight the need for improved tools for detecting and surveilling novel viral pathogens.
This panel's design incorporates the use of the CATCH (Compact Aggregation of Targets for Comprehensive Hybridization) algorithm. For additional details on CATCH, please refer to Metsky HC and Siddle KJ et al. Capturing sequence diversity in metagenomes with comprehensive and scalable probe design. Nature Biotechnology, 37(2), 160–168 (2019). doi: 10.1038/ s41587-018-0006-x
The Twist Comprehensive Viral Research Panel covers reference sequences for 3,153 viruses, including 15,488 different strains. Examples of viruses that can be detected include:
Adenoviruses
Coronaviruses
Herpesviruses
Influenza A & B
Mpox
Noroviruses
Papillomaviruses
Parvoviruses
Picornaviruses
Poxviruses
Retroviruses
Rhabdoviruses
See all families here
Discover New Viral Species
The pandemic emergence of SARS-CoV-2 along with the mpox outbreak highlight the need for improved tools for detecting and surveilling novel viral pathogens.
The Twist Comprehensive Viral Research Panel covers reference sequences for 3,153 viruses, including 15,488 different strains. Examples of viruses that can be detected include:
Adenoviruses
Coronaviruses
Herpesviruses
Influenza A & B
Mpox
Noroviruses
Papillomaviruses
Parvoviruses
Picornaviruses
Poxviruses
Retroviruses
Rhabdoviruses
See all families here
This panel's design incorporates the use of the CATCH (Compact Aggregation of Targets for Comprehensive Hybridization) algorithm. For additional details on CATCH, please refer to Metsky HC and Siddle KJ et al. Capturing sequence diversity in metagenomes with comprehensive and scalable probe design. Nature Biotechnology, 37(2), 160–168 (2019). doi: 10.1038/ s41587-018-0006-x
We pulled reference sequences from the RefSeq, FluDB, and VIPR databases to design the 1,052,421 unique probes that make up the Twist Comprehensive Viral Research Panel.
The panel broadly spans every viral family that contains at least one virus known to affect humans, including adenoviruses, coronaviruses, flaviviruses, noroviruses, influenza viruses, and more.
In addition to targeting human-infective viruses, the panel also covers animal viruses (e.g., bat coronaviruses). This enables the discovery of novel viral species regardless of origin.
Its sheer breadth allows the Twist Comprehensive Viral Research Panel to detect novel and evolved viral strains, including those related to a June 2020 outbreak of H1N1 influenza in swine workers.
![Comprehensive Novel Virus Detection](/sites/default/files/2020-11/NGS-Fixed-Panel-Comp-Virus-Research-Tree-2.png)
Natural selection of coronavirus spike and influenza hemagglutinin (HA) glycoproteins in animals facilitated the zoonotic transmission of SARS-CoV-2 and H1N1 influenza to humans.
Here, we show that the Twist Comprehensive Viral Research Panel can be used to successfully detect these hard-to-capture sequences, including the spike region of a recently discovered coronavirus (RoBat-CoV GCCDC1 Singapore) and a HA segment isolated from a June 2020 outbreak of H1N1 influenza in swine workers.
Our panel resulted in >99.8% coverage of each sequence to at least 1x depth.
![Detect highly divergent viral sequences](/sites/default/files/2020-11/NGS-Fixed-Panel-Comp-Virus-Research-Probes.png)
We defined the mismatch sensitivity of the Twist Comprehensive Viral Research Panel by generating a series of HA segments to contain defined levels of sequence variation ranging from 5% to 30% variation from a wild-type HA segment.
Total capture was achieved at 1x coverage in samples with up to 10% sequence variation. Together, these data demonstrate that the Twist Comprehensive Viral Research Panel can capture highly evolved viral sequences.
Multiplex detection of viruses is possible with the Twist Comprehensive Viral Research Panel, enabling metagenomic applications in a variety of sample types.
In a co-infection assay, the panel successfully captured four different virus types (Astrovirus - ssRNA, BK virus - dsDNA, Bocavirus - ssDNA and Picobirnavirus - dsRNA) spiked into human RNA with only 1.2 M reads.
We pulled reference sequences from the RefSeq, FluDB, and VIPR databases to design the 1,052,421 unique probes that make up the Twist Comprehensive Viral Research Panel.
The panel broadly spans every viral family that contains at least one virus known to affect humans, including adenoviruses, coronaviruses, flaviviruses, noroviruses, influenza viruses, and more.
In addition to targeting human-infective viruses, the panel also covers animal viruses (e.g., bat coronaviruses). This enables the discovery of novel viral species regardless of origin.
Its sheer breadth allows the Twist Comprehensive Viral Research Panel to detect novel and evolved viral strains, including those related to a June 2020 outbreak of H1N1 influenza in swine workers.
![Comprehensive Novel Virus Detection](/sites/default/files/2020-11/NGS-Fixed-Panel-Comp-Virus-Research-Tree-2.png)
Natural selection of coronavirus spike and influenza hemagglutinin (HA) glycoproteins in animals facilitated the zoonotic transmission of SARS-CoV-2 and H1N1 influenza to humans.
Here, we show that the Twist Comprehensive Viral Research Panel can be used to successfully detect these hard-to-capture sequences, including the spike region of a recently discovered coronavirus (RoBat-CoV GCCDC1 Singapore) and a HA segment isolated from a June 2020 outbreak of H1N1 influenza in swine workers.
Our panel resulted in >99.8% coverage of each sequence to at least 1x depth.
![Detect highly divergent viral sequences](/sites/default/files/2020-11/NGS-Fixed-Panel-Comp-Virus-Research-Probes.png)
We defined the mismatch sensitivity of the Twist Comprehensive Viral Research Panel by generating a series of HA segments to contain defined levels of sequence variation ranging from 5% to 30% variation from a wild-type HA segment.
Total capture was achieved at 1x coverage in samples with up to 10% sequence variation. Together, these data demonstrate that the Twist Comprehensive Viral Research Panel can capture highly evolved viral sequences.
Multiplex detection of viruses is possible with the Twist Comprehensive Viral Research Panel, enabling metagenomic applications in a variety of sample types.
In a co-infection assay, the panel successfully captured four different virus types (Astrovirus - ssRNA, BK virus - dsDNA, Bocavirus - ssDNA and Picobirnavirus - dsRNA) spiked into human RNA with only 1.2 M reads.
The Twist Comprehensive Viral Research Panel includes access to the web analysis tool One Codex. In addition to supplying analysis support, this cloud-based tool allows users to document their novel virus discovery efforts with printable reports and publication-ready figures.
One Codex simplifies the analysis of sequence data obtained using the Twist Comprehensive Viral Research Panel. After uploading sequence data with a straightforward drag-and-drop interface, users can generate a report detailing their novel virus discovery results within minutes. Users can also customize the bioinformatics workflow with their own detection thresholds and interpretations.
Purchase of the Twist Comprehensive Viral Research Panel comes with everything needed to start analyzing your results with One Codex, including a coupon code and detailed instructions. Learn more about this streamlined microbial analysis platform at onecodex.com
![One Codex Analysis](/sites/default/files/2020-11/NGS-Respiratory-Viral-Controls-One-Codex-2_0.png)
The Twist Comprehensive Viral Research Panel includes access to the web analysis tool One Codex. In addition to supplying analysis support, this cloud-based tool allows users to document their novel virus discovery efforts with printable reports and publication-ready figures.
One Codex simplifies the analysis of sequence data obtained using the Twist Comprehensive Viral Research Panel. After uploading sequence data with a straightforward drag-and-drop interface, users can generate a report detailing their novel virus discovery results within minutes. Users can also customize the bioinformatics workflow with their own detection thresholds and interpretations.
Purchase of the Twist Comprehensive Viral Research Panel comes with everything needed to start analyzing your results with One Codex, including a coupon code and detailed instructions. Learn more about this streamlined microbial analysis platform at onecodex.com
![One Codex Analysis](/sites/default/files/2020-11/NGS-Respiratory-Viral-Controls-One-Codex-2_0.png)
103545
Twist Comprehensive Viral Research Panel with One Codex software, 2 Reactions, Kit103547
Twist Comprehensive Viral Research Panel with One Codex software, 12 Reactions, Kit103548
Twist Comprehensive Viral Research Panel with One Codex software, 96 Reactions, KitWorkflow Step
Target EnrichmentFormat
dsDNAPanel size
36.8 MbContent
Viral sequencesDesign Databases
FluDB, RefSeq, VIPRdbStorage
Store at -5 to -30C103545
Twist Comprehensive Viral Research Panel with One Codex software, 2 Reactions, Kit103547
Twist Comprehensive Viral Research Panel with One Codex software, 12 Reactions, Kit103548
Twist Comprehensive Viral Research Panel with One Codex software, 96 Reactions, KitWorkflow Step
Target EnrichmentFormat
dsDNAPanel size
36.8 MbContent
Viral sequencesDesign Databases
FluDB, RefSeq, VIPRdbStorage
Store at -5 to -30C
Application Note
NGS Target Enrichment of Viral Pathogens using Twist Respiratory Virus Research Panel