A better understanding how the fate of T cells is controlled will further our knowledge of adaptive immunity, which in turn could yield insights into how we respond to pathogens, allergens and tumors. Join us for this Twist-hosted webinar with Dr. Hongling Huang, Professor, School of Life Sciences, Xiamen University, to learn how they have been using in vivo CRISPR screens to reveal regulators of T cell fate decisions. Discover how Twist’s Oligo Pools paves the way for the next generation CRISPR screens and enables limitless discoveries in your research.

CRISPR knock-out screens provide a powerful tool with which to investigate highly coordinated cellular processes. Using genome-wide CRISPR knockout screens, followed by focused CRISPR screens, researchers at Boehringer Ingelheim probed the gene-phenotype relationship of human monocyte phagocytosis when the cells were challenged with Staphylococcus aureus. The focused CRISPR screen, which used a custom high-quality guide library synthesized on Twist Bioscience’s DNA synthesis platform, cut the number of candidate genes that needed to be independently tested by 90%, saving Boehringer Ingelheim significant resources throughout their experiment.

Discovery of Synthetic Lethal Paralog Pairs in the Human Genome