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When the Nebraska Public Health Laboratory (NPHL) learned that travelers potentially exposed to Andes virus were arriving within days, the team faced an unusual challenge: develop and validate an RT-qPCR assay for a rare pathogen in less than 72 hours. Publicly shared genomic data gave researchers the sequence information they needed, but validating the assay required control material that closely matched the strain associated with the outbreak. For this, they turned to Twist for help.