Genes, planes, and automobiles: How the Nebraska Public Health Laboratory developed a hantavirus assay in 72 hours

When the Nebraska Public Health Laboratory (NPHL) learned that travelers potentially exposed to Andes virus were arriving within days, the team faced an unusual challenge: develop and validate an RT-qPCR assay for a rare pathogen in less than 72 hours. Publicly shared genomic data gave researchers the sequence information they needed, but validating the assay required control material that closely matched the strain associated with the outbreak. For this, they turned to Twist for help.


Covered in this case study
How the NPHL team developed an RT-qPCR assay for Andes virus in less than 72 hours
The value of strain-matched synthetic control material in viral testing
How Twist ensured the NPHL team could validate their assay on such a short timeline
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