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Viral Detection and Characterization: Using the Twist Fast Hybridization Target Enrichment Protocol
Here, the FastHyb workflow is fine-tuned for rapid capture, and optimal detection of SARS-CoV-2 RNA by NGS.
Twist’s Fast Hybridization Target Enrichment system is designed with the flexibility to both save time and enhance the performance of targeted sequencing efforts. Both time and process stringency can be tuned to fit the needs of the investigator.Here we show the Twist Fast Hybridization system’s optimal Fast Wash Buffer 1 temperature, its flexibility in hybridization time, and its compatibility with 8-plex hybridization for detecting and characterizing viral genomes using the Twist SARS-CoV-2 Research Panel.
Covered in this Application Note
Optimization of wash buffer temperature
Flexibility of hybridization time depending on experimental needs.
Use of multiplexing for viral detection
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