Hyperimmune Library Series

The Twist Hyperimmune Library synthetically mimics the human in vivo antibody repertoire, providing optimal diversity for antibody development against any target.

This naïve library is the original design of the Twist Hyperimmune Library series, which also includes the VHH hShuffle Hyperimmune (HI) and Common Light Chain Hyperimmune libraries. 

Hyperimmune Fab Library

A highly diverse kappa VK1-39 light chain library was paired with the VH3-23 heavy chain library to yield a highly diverse fully human Fab phage display library.

Hyperimmune scFv Library

HCDR3 sequence diversities were combinatorially assembled and incorporated into the humanized VH3-23 framework to construct the heavy chain library, which was then paired with a highly diverse kappa VK1-39 light chain library. This combination yields a highly functional antibody library of ~10¹⁰ size. Originally offered as a Fab library, the library is now also available in the more compact scFv format. 

VHH hShuffle Hyperimmune Library

Offers high diversity human CDR3 with natural llama CDR1/2 sequences in a humanized DP-47 VHH framework. More than two million HCDR3 sequences were gathered from a database of human naïve and memory B-cell receptor sequences and incorporated into the VHH hShuffle library 

Hyperimmune Common Light Chain Library

For the common light chain hyperimmune library, trastuzumab light chain is cloned in place of the VK1-39 light chain library.