Minotaur scFv Library

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OVERVIEW
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Access Hidden Epitopes with Ultralong HCDR3

The Twist Minotaur scFv Library is a new synthetic antibody library that grafts ultralong HCDR3 loops from bovine antibodies into a fully human antibody framework. These elongated HCDR3 loops protrude far from the antibody surface, enabling access to occluded epitopes within protein crevices, pores, and channels. This unique library can empower your therapeutic antibody discovery and development for any indication.

Be among the first to access the synthetic advantage for the discovery of antibodies against general and hard-to-drug targets like GPCRs, ion channels, and other membrane-bound proteins.

Produce scFv antibodies against challenging epitopes

Fully human antibody framework sequences
Proven, highly manufacturable framework with development liabilities removed
Two sub libraries of >109 diversity

Explore challenging epitopes with ultralong bovine HCDR3 loops

Ideal for targeting hard-to-reach epitopes
Ultralong HCDR3 loops up to 60 amino acids in length
Defined cysteine mutations replicate bovine antibody diversification

Synthetic library advantage

Panning to functional assays in 10-12 weeks without immunization
Focus on effective sequence space
Screen multiple targets simultaneously

Library Specifications

The Twist Minotaur scFv Library incorporates ultralong bovine HCDR3s into the VH3-23/VK1-39 human antibody framework. The ultralong HCDR3 loops are up to 60 amino acids in length, making them three- to fourfold longer than the average human CDR3 loop.

The Twist Minotaur scFv Library includes a set of two sublibraries, each of which incorporates diversity from the Twist Hyperimmune Original Fab Library (HCDR1, HCDR2, LCDR1, LCDR2, and LCDR3) and a proprietary bovine antibody database (HCDR3). Both sublibraries are provided as a part of the Minotaur scFv library and can be panned in parallel against your target of interest.

Sublibrary 1 introduces ultralong bovine HCDR3 loops with an even number of up to ten cysteines to promote the formation of stabilizing disulfide bonds, diversifying the library repertoire. The diversity of Sublibrary 1 is 6 x 109.

Sublibrary 2 contains an odd number of up to nine cysteines in ultralong HCDR3 and another cysteine in HCDR2 or the human antibody framework. By mimicking what is observed spatially in native bovine antibodies, Sublibrary 2 offers three cysteine-mutated versions of the human framework to foster disulfide bond formation with the sole cysteine in the HCDR3 loop. The diversity of Sublibrary 2 is 3.6 x 109.

Library Panning & Screening Process

Go from panning to functional assays in 10–12 weeks. The process starts with phage screening the diverse Twist Minotaur scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Minotaur scFv library to initiate your own in-house discovery projects. To learn more, get in touch at biopharma@twistbioscience.com